Intra-amniotic Injection of Mouse Embryos
| Autor: | Peng Zou, Jiayi Zhang, Liyuan Cui, Lu Lu, Qingshuo Meng |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Retina
Amniotic fluid biology business.industry Strategy and Management Mechanical Engineering Metals and Alloys Outbreak Physiology Embryo biology.organism_classification Industrial and Manufacturing Engineering Embryo transfer Virus Zika virus medicine.anatomical_structure Methods Article Intra-amniotic injection medicine business |
| Zdroj: | Bio Protoc |
| ISSN: | 2331-8325 |
| DOI: | 10.21769/bioprotoc.2854 |
| Popis: | Recent outbreaks of infectious neuro-developmental diseases such as congenital Zika syndrome - have led to a demand for prognosis data from animal models. We developed an intra-amniotic injection mice model that allows Zika virus (ZIKV) infected mice to grow to puberty. In this system, ZIKV is injected into the amniotic fluid of pregnant mice and infected embryos thereafter. ZIKV-infected mice show several symptoms of clinical ‘congenital Zika syndrome’, including decreased brain volume and mis-laminated retina. We also evaluated several behavioral functions of these ZIKV-infected mice, for example, after the mice reach puberty, they have visual and motor defects. This technique can be used to screen and evaluate drug candidates and may help evaluate the prognosis of infectious neuro-developmental diseases. |
| Databáze: | OpenAIRE |
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