Isolation and Characterization of the Human Tissue Transglutaminase Gene Promoter
| Autor: | Peter J.A. Davies, Margaret Saydak, Shan Lu, Vittorio Gentile, Joseph P. Stein |
|---|---|
| Přispěvatelé: | Lu, S, Saydak, M, Gentile, Vittorio, Stein, Jp, Davies, Pj |
| Rok vydání: | 1995 |
| Předmět: |
Sp1 Transcription Factor
Tissue transglutaminase TATA box Guinea Pigs Molecular Sequence Data Response element CAAT box Biology Biochemistry Mice chemistry.chemical_compound Transcription (biology) Sequence Homology Nucleic Acid Tumor Cells Cultured Animals Humans Amino Acid Sequence Promoter Regions Genetic Molecular Biology Gene Transglutaminases Base Sequence Promoter 3T3 Cells DNA Cell Biology Molecular biology chemistry biology.protein |
| Zdroj: | Journal of Biological Chemistry. 270:9748-9756 |
| ISSN: | 0021-9258 |
| DOI: | 10.1074/jbc.270.17.9748 |
| Popis: | Tissue transglutaminase belongs to a family of calcium-dependent enzymes, the transglutaminases that catalyze the covalent cross-linking of specific proteins by the formation of epsilon (gamma-glutamyl)lysine isopeptide bonds. The goal of this study has been the isolation and characterization of the human tissue transglutaminase gene promoter. Genomic DNA clones, spanning the 5' region of the gene, were isolated and the structure of the 5'-end of the human tissue transglutaminase gene was determined. 1.74 kilobases of flanking DNA were sequenced and were found to contain a TATA box element (TATAA), a CAAT box element (GGACAAT), a series of potential transcription factor-binding sites (AP1, SP1, interleukin-6 response element), and a glucocorticoid response elements. Transient transfection experiments showed that this DNA fragment included a functional promoter, which is constitutively active in multiple cell types. |
| Databáze: | OpenAIRE |
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