| Autor: |
Jason T Ladner, Sierra N Henson, Annalee S Boyle, Anna L Engelbrektson, Zane W Fink, Fatima Rahee, Jonathan D’ambrozio, Kurt E Schaecher, Mars Stone, Wenjuan Dong, Sanjeet Dadwal, Jianhua Yu, Michael A Caligiuri, Piotr Cieplak, Magnar Bjørås, Mona H Fenstad, Svein A Nordbø, Denis E Kainov, Norihito Muranaka, Mark S Chee, Sergey A Shiryaev, John A Altin |
| Rok vydání: |
2020 |
| Předmět: |
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| Zdroj: |
bioRxiv : the preprint server for biology. |
| Popis: |
A high-resolution understanding of the antibody response to SARS-CoV-2 is important for the design of effective diagnostics, vaccines and therapeutics. However, SARS-CoV-2 antibody epitopes remain largely uncharacterized, and it is unknown whether and how the response may cross-react with related viruses. Here, we use a multiplexed peptide assay (‘PepSeq’) to generate an epitope-resolved view of reactivity across all human coronaviruses. PepSeq accurately detects SARS-CoV-2 exposure and resolves epitopes across the Spike and Nucleocapsid proteins. Two of these represent recurrent reactivities to conserved, functionally-important sites in the Spike S2 subunit, regions that we show are also targeted for the endemic coronaviruses in pre-pandemic controls. At one of these sites, we demonstrate that the SARS-CoV-2 response strongly and recurrently cross-reacts with the endemic virus hCoV-OC43. Our analyses reveal new diagnostic and therapeutic targets, including a site at which SARS-CoV-2 may recruit common pre-existing antibodies and with the potential for broadly-neutralizing responses. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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