Two-Dimensional Crystals of Protein Kinase C
| Autor: | Robert H. Kretsinger, Jonathan M. Owens, Olga Chertihin, Julianne J. Sando |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Protein Kinase C-alpha
Protein Conformation Chemistry Spectrum Analysis X-Rays Phosphatidylserines Recombinant Proteins Phorbol ester Diglycerides Isoenzymes Crystal Microscopy Electron Protein Kinase C-delta Crystallography Structural Biology Molar ratio Electron micrographs Monolayer Image Processing Computer-Assisted Phosphatidylcholines Phosphorylation Crystallization Protein Kinase C Protein kinase C C2 domain |
| Zdroj: | Journal of Structural Biology. 121:61-67 |
| ISSN: | 1047-8477 |
| Popis: | Three two-dimensional (2D) crystal forms of protein kinase C (PKC) alpha and three of PKC delta have been grown on lipid monolayers composed of dioleoylphosphatidylcholine: dioleoylphosphatidylserine: (45:50:5 molar ratio). In the absence of DO, two additional 2D crystals of PKC delta are seen, suggesting that the presence of diolein (DO) alters the conformation of intact PKC at the lipid surface. Reconstructions of electron micrographs of these eight lattices show good reproducibility and indicate that several are appropriate for three-dimensional reconstruction to 20 A resolution. |
| Databáze: | OpenAIRE |
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