Determination of Moxidectin in Serum by Liquid Chromatography-Tandem Mass Spectrometry and Its Application in Pharmacokinetic Study in Lambs
| Autor: | Maria Angela Machado Fernandes, Alda Lúcia Gomes Monteiro, Sonia C. N. Queiroz, Rafaela C. Baptista, Felix Guillermo Reyes Reyes, Vera Lúcia Ferracini, Susana Gilaverte, Márcia Regina Assalin |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Detection limit
Chromatography Coefficient of variation 010401 analytical chemistry Extraction (chemistry) 0402 animal and dairy science 04 agricultural and veterinary sciences General Chemistry veterinary drug Mass spectrometry 040201 dairy & animal science 01 natural sciences 0104 chemical sciences Moxidectin chemistry.chemical_compound chemistry Pharmacokinetics Liquid chromatography–mass spectrometry serum lamb Veterinary drug moxidectin pharmacokinetic LC-MS/MS |
| Zdroj: | Journal of the Brazilian Chemical Society v.28 n.2 2017 Journal of the Brazilian Chemical Society Sociedade Brasileira de Química (SBQ) instacron:SBQ Journal of the Brazilian Chemical Society, Volume: 28, Issue: 2, Pages: 250-256, Published: FEB 2017 |
| Popis: | The widespread use of moxidectin (MOX), a parasiticide used in the sheep breeding, has induced the parasite resistance in Brazilian farms. As a consequence, the farmers often increase the dose and frequency of drug utilization, and disregards safety of meat or milk. In order to establish adequate therapeutic treatment it is necessary to know the pharmacokinetics of the drug in the animal's body. Thus, high performance liquid chromatography-electrospray ionization-tandem mass spectrometry (HPLC-ESI-MS/MS) method was developed for the determination of MOX in serum lamb. Serum samples were treated with acetonitrile to precipitate proteins. A clean up by dispersive extraction in solid phase (SPE-d), using primary/secondary amine (PSA) and C18 sorbents, followed by freezing was performed. Method validation presented precision (coefficient of variation) and accuracy (recovery%) between 1.7-6.7 and 80.0-107.3%, respectively. The limit of quantification (LOQ) of the method was 2.0 ng mL-1 and a linear response was obtained over a range of 2.0 to 100 ng mL-1. This method was successfully applied to the determination of MOX in serum from suffolk lamb to evaluate the pharmacokinetic profile. |
| Databáze: | OpenAIRE |
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