The effect of an acute elevation of NEFA concentrations on glucagon-stimulated hepatic glucose output
| Autor: | Suzan Vaughan, E. Patrick Donahue, Carrie A. Everett-Grueter, Chang An Chu, Alan D. Cherrington, Dale S. Edgerton, Dana K. Sindelar |
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| Rok vydání: | 2006 |
| Předmět: |
Blood Glucose
Glycerol medicine.medical_specialty Fat Emulsions Intravenous Hepatic glucose Glycogenolysis Physiology Endocrinology Diabetes and Metabolism Carbohydrate metabolism Fatty Acids Nonesterified Glucagon Acetoacetates NEFA Dogs Physiology (medical) Internal medicine medicine Cyclic AMP Animals Insulin Lactic Acid Amino Acids 3-Hydroxybutyric Acid Chemistry Heparin Gluconeogenesis Glucose clamp technique Liver metabolism Endocrinology Glucose Liver Hyperglycemia Glucose Clamp Technique |
| Zdroj: | American journal of physiology. Endocrinology and metabolism. 291(3) |
| ISSN: | 0193-1849 |
| Popis: | To determine the effect of nonesterified fatty acids (NEFA) on glucagon action, glucagon was infused intraportally (1.65 ng·min−1·kg−1) for 3 h into 18-h-fasted, pancreatic-clamped conscious dogs in the presence [NEFA + glucagon (GGN)] or absence (GGN) of peripheral Intralipid plus heparin infusion. Additionally, hyperglycemic (HG), hyperglycemic-hyperlipidemic (NEFA + HG), and glycerol plus glucagon (GLYC + GGN) controls were studied. Arterial plasma glucagon concentrations rose equally in GGN, NEFA + GGN, and GLYC + GGN but remained basal in hyperglycemic controls. Peripheral infusions of Intralipid and heparin increased arterial plasma NEFA concentrations equally in NEFA + GGN and NEFA + HG and did not change in other protocols. After 15 min, glucagon infusion resulted in a rapid, brief increase in net hepatic glycogenolysis (NHGLY, mg·min−1·kg−1) of ∼6.0 in GGN and GLYC + GGN but only increased by 3.8 ± 1.3 in NEFA + GGN. Thus increases in NHGLY, and consequently net hepatic glucose output (NHGO), were blunted by 40%, with no difference between the groups in the last 2.5 h of the study. NHGO and NHGLY did not significantly change in HG and NEFA + HG. Net hepatic gluconeogenic flux did not change in GGN, GLYC + GGN, or HG. However, Intralipid and heparin infusion resulted in similar increases in net hepatic gluconeogenic flux in NEFA + GGN and NEFA + HG. Thus elevated NEFA limit the initial increase in glucagon-stimulated HGO by blunting glycogenolysis, without having any effect on the gluconeogenic or glycogenolytic contributions or NHGO thereafter. |
| Databáze: | OpenAIRE |
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