Nuclear envelope-vacuole contacts mitigate nuclear pore complex assembly stress
| Autor: | Susan R. Wente, Christopher L. Lord |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0303 health sciences
Atg1 biology Chemistry Saccharomyces cerevisiae Mutant Vacuole biology.organism_classification Cell biology 03 medical and health sciences stomatognathic diseases 0302 clinical medicine Lipid droplet otorhinolaryngologic diseases Nuclear pore Nuclear transport 030217 neurology & neurosurgery Biogenesis 030304 developmental biology |
| DOI: | 10.1101/2020.03.23.001719 |
| Popis: | The intricacy of nuclear pore complex (NPC) biogenesis imposes risks of failure that can cause defects in nuclear transport and nuclear envelope morphology, however, cellular mechanisms utilized to alleviate NPC assembly stress are not well-defined. In the budding yeast Saccharomyces cerevisiae, we demonstrate that NVJ1- and MDM1-enriched nuclear envelope (NE)-vacuole contacts increase when NPC assembly is compromised in several nup mutants, including nup116ΔGLFG cells. These interorganelle nucleus-vacuole junctions (NVJs) cooperate with lipid droplets to maintain viability and enhance NPC formation in assembly mutants. Additionally, NVJs function with ATG1 to promote vacuole-dependent remodeling in nup116ΔGLFG cells, which also correlates with proper NPC formation. Importantly, NVJs significantly improve the physiology of NPC assembly mutants, despite having only negligible effects when NPC biogenesis is unperturbed. Collectively, these results define how NE-vacuole interorganelle contacts coordinate responses to mitigate deleterious cellular effects caused by disrupted NPC assembly.SummaryHow cells respond to deleterious effects imposed by disrupted nuclear pore complex (NPC) assembly are not well-defined. The authors demonstrate nuclear envelope-vacuole interactions expand in response to perturbed NPC assembly to promote viability, nuclear envelope remodeling, and proper NPC biogenesis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|