Diversity within the genus Elymus (Poaceae: Triticeae) as investigated by the analysis of the nr5S rDNA variation in species with St and H haplomes
| Autor: | Douglas A. Johnson, Bernard R. Baum, T. Edwards |
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| Rok vydání: | 2014 |
| Předmět: |
Elymus
Population DNA Ribosomal Analysis of molecular variance Polyploidy Species Specificity Phylogenetics Genus Botany Genetics Triticeae education Molecular Biology Phylogeny Principal Component Analysis education.field_of_study Base Sequence Models Genetic Phylogenetic tree biology Genetic Variation Species diversity Bayes Theorem General Medicine biology.organism_classification Genetics Population Haplotypes Evolutionary biology |
| Zdroj: | Molecular Genetics and Genomics. 290:329-342 |
| ISSN: | 1617-4623 1617-4615 |
| Popis: | The genus Elymus ("Ryegrass") is a repository for a range of species with a variety of haplome contents; hence the pejorative name "dustbin" genus. We have analyzed 1,059 sequences from 128 accessions representing 24 species to investigate the relationships among the StH haplomes-containing species described by Yen and Yang (Genus Elymus Beijing 5:58-362, 2013). Sequences were assigned to "unit classes" of orthologous sequences and subjected to a suite of analyses including BLAST (Basic Local Alignment Search Tool) searches, phylogenetic analysis and population genetic analysis to estimate species diversity. Our results support the genome analyses in Yen and Yang (Genus Elymus Beijing 5:58-362, 2013), i.e., genomic constitution StStHH including variants restricted to Elymus. Population genetic analysis of the 5S nrDNA sequence data revealed that the within-species variance component is roughly ±89 %; thus, we were unable to identify molecular markers capable to separate the 24 species analyzed. Separate phylogenetic analyses of the two unit classes and of all the data exhibit a trend only of the species to cluster on the phylograms. Finally, the analysis provides evidence for the multiple origins of American and Eurasian species. |
| Databáze: | OpenAIRE |
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