Evidence of an auxin-mediated phosphoinositide turnover and an inositol (1,4,5)trisphosphate effect on isolated membranes ofDaucus carota L
| Autor: | Bernd A. Zbell, Hubert Bucher, Cornelia Walter-Back |
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| Rok vydání: | 1989 |
| Předmět: |
Inositol Phosphates
Phospholipid Inositol 1 4 5-Trisphosphate Biology Phosphatidylinositols Biochemistry chemistry.chemical_compound Plant Growth Regulators Auxin Microsomes Inositol Phosphorylation Lipid phosphorylation Molecular Biology chemistry.chemical_classification Indoleacetic Acids Cell Membrane food and beverages Cell Biology Plants Inositol trisphosphate receptor Lipids Kinetics Membrane chemistry Calcium Sugar Phosphates Signal transduction |
| Zdroj: | Journal of Cellular Biochemistry. 40:331-340 |
| ISSN: | 1097-4644 0730-2312 |
| DOI: | 10.1002/jcb.240400309 |
| Popis: | Microsomal membranes from carrot suspension cells were phosphorylated in vitro with [gamma-32P]ATP. In the presence of submicromolar concentrations of the natural auxin indoleacetic acid (IAA), a rapid, but transient decrease of the [32P] label could be detected in the phospholipid extracts of the membranes. The phytohormone effect was not the result of an inhibition of the lipid phosphorylation reactions, but was caused by a simultaneous release of water-soluble compounds, which, according to their chromatographic properties, were assumed to contain inositol polyphosphates. Although the [32P]-labeled lipids, as well as the inositol polyphosphates, were not identified unequivocally by chemical analysis, these findings point to an auxin-mediated control of a phosphoinositidase C-like reaction similar to the hormone-stimulated phosphoinositide response in animals. Exogenously applied inositol (1,4,5)trisphosphate [(1,4,5)IP3] was found to release 45Ca2+ from preloaded membrane vesicles of carrot cells. Both the detection of the auxin-stimulated phosphoinositide response and the (1,4,5)IP3-mediated Ca2+ release on isolated cell membranes offer new experimental approaches for the identification of the putative auxin receptor and its signal transduction pathway. |
| Databáze: | OpenAIRE |
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