Quantifying Protein-Specific N-Glycome Profiles by Focused Protein and Immunoprecipitation Glycomics
| Autor: | Goki Suda, Takuya Sho, Tomoyo Yoshinaga, Megumi Hato, Koji Ogawa, Yasuro Shinohara, Kenichi Higashino, Takashi Kobayashi, Kenichi Morikawa, Yoshito Numata, Jun-ichi Furukawa, Masato Nakai, Naoya Sakamoto, Hisatoshi Hanamatsu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Male Glycan Glycosylation Immunoprecipitation Biochemistry glycomics Glycomics 03 medical and health sciences Non-alcoholic Fatty Liver Disease Polysaccharides medicine Humans MALDI-TOF MS Biomarker discovery 030102 biochemistry & molecular biology biology Chemistry Fatty liver NASH General Chemistry Blood Proteins Middle Aged medicine.disease Glycome Blood proteins ceruloplasmin carbohydrates (lipids) 030104 developmental biology Liver Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization alpha 1-Antitrypsin biology.protein fucosylation Biomarker (medicine) biomarker Female alpha-1 antitrypsin Biomarkers |
| Zdroj: | Journal of proteome research. 18(8):3133-3141 |
| ISSN: | 1535-3893 |
| Popis: | Serum N-glycans have been reported to be potential diagnostic and therapeutic biomarkers for many diseases and conditions, such as inflammation, fibrosis, and cancer progression. We previously described the focused protein glycomic analysis (FPG) from gel-separated serum proteins. With this methodology, we sought novel glycan biomarkers for nonalcoholic steatohepatitis (NASH) and successfully identified some N-glycans that were significantly elevated in NASH patients compared to nonalcoholic fatty liver patients. Among them, trisialylated monofucosylated triantennary glycan (A3F) of alpha-1 antitrypsin showed the most dynamic change. For rapid identification of N-glycans on the focused proteins, we constructed a simplified method called immunoprecipitation glycomics (IPG), where the target proteins were immunoprecipitated with affinity beads and subsequently subjected to glycomic analysis by MALDI-TOF MS. Focusing on alpha-1 antitrypsin and ceruloplasmin as the target proteins, we compared the values of N-glycans determined by FPG and IPG. The quantified values of each N-glycan by these two methods showed a statistically significant correlation, indicating that high throughput and quantitative N-glycomics of targeted proteins can be achieved by the simplified IPG method. Thus, an analytical strategy combining FPG and IPG can be adapted to general biomarker discovery and validation in appropriate disease areas. |
| Databáze: | OpenAIRE |
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