Post-Synthetic Reduction of Pectin Methylesterification Causes Morphological Abnormalities and Alterations to Stress Response in Arabidopsis thaliana
| Autor: | Diane C. Bassham, Song Gao, Lauran Chambers, Ning Zhang, Olga A. Zabotina, Charles T. Anderson, Junmarie Soto-Burgos, Justin W. Walley, Guanhua Feng, Gennady Pogorelko, Yintong Chen, Nathan T. Reem, Siti Farah Abdullah |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Arabinose food.ingredient Arabidopsis thaliana Osmotic shock Pectin Plant Science 01 natural sciences Article pattern-triggered immunity Cell wall Botrytis cinerea 03 medical and health sciences chemistry.chemical_compound pectin methylesterification food Aspergillus nidulans lcsh:Botany Pectinase Ecology Evolution Behavior and Systematics Ecology biology fungi food and beverages biology.organism_classification Plant cell lcsh:QK1-989 Cell biology 030104 developmental biology chemistry cell wall signaling 010606 plant biology & botany |
| Zdroj: | Plants, Vol 9, Iss 1558, p 1558 (2020) Plants Volume 9 Issue 11 |
| ISSN: | 2223-7747 |
| Popis: | Pectin is a critical component of the plant cell wall, supporting wall biomechanics and contributing to cell wall signaling in response to stress. The plant cell carefully regulates pectin methylesterification with endogenous pectin methylesterases (PMEs) and their inhibitors (PMEIs) to promote growth and protect against pathogens. We expressed Aspergillus nidulans pectin methylesterase (AnPME) in Arabidopsis thaliana plants to determine the impacts of methylesterification status on pectin function. Plants expressing AnPME had a roughly 50% reduction in methylester content compared with control plants. AnPME plants displayed a severe dwarf phenotype, including small, bushy rosettes and shorter roots. This phenotype was caused by a reduction in cell elongation. Cell wall composition was altered in AnPME plants, with significantly more arabinose and significantly less galacturonic acid, suggesting that plants actively monitor and compensate for altered pectin content. Cell walls of AnPME plants were more readily degraded by polygalacturonase (PG) alone but were less susceptible to treatment with a mixture of PG and PME. AnPME plants were insensitive to osmotic stress, and their susceptibility to Botrytis cinerea was comparable to wild type plants despite their compromised cell walls. This is likely due to upregulated expression of defense response genes observed in AnPME plants. These results demonstrate the importance of pectin in both normal growth and development, and in response to biotic and abiotic stresses. |
| Databáze: | OpenAIRE |
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