Mechanism of growth inhibition by MicroRNA 145: The role of the IGF-I receptor signaling pathway
| Autor: | Bin Shi, Laura Sepp-Lorenzinoi, Margherita Badin, Tiziana DeAngelis, Renato Baserga, Shi-Qiong Xu, Gaspare La Rocca |
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| Rok vydání: | 2009 |
| Předmět: |
Untranslated region
Physiology Colorectal cancer Clinical Biochemistry Cell Active Transport Cell Nucleus Receptor IGF Type 1 Mice chemistry.chemical_compound Growth factor receptor Cell Line Tumor microRNA medicine Animals Humans 3' Untranslated Regions biology 3T3 Cells Cell Biology medicine.disease Cell biology Gene Expression Regulation Neoplastic MicroRNAs Insulin receptor medicine.anatomical_structure chemistry Colonic Neoplasms Insulin Receptor Substrate Proteins biology.protein Growth inhibition Signal transduction Signal Transduction |
| Zdroj: | Journal of Cellular Physiology. 220:485-491 |
| ISSN: | 1097-4652 0021-9541 |
| Popis: | MicroRNA 145 (miR145) has been proposed as a tumor suppressor. It was previously shown that miR145 targets the 3′ UTR of the insulin receptor substrate-1 (IRS-1) and dramatically inhibits the growth of colon cancer cells. miR145 also targets the type 1 insulin-like growth factor receptor (IGF-IR). We show here that an IRS-1 lacking its 3′ UTR is no longer down-regulated by miR145 and rescues colon cancer cells from miR145-induced inhibition of growth. An IGF-IR resistant to miR145 (again by elimination of its 3′ UTR) is not down-regulated by miR145 but fails to rescue colon cancer cells from growth inhibition. These and other results, taken together, indicate that down-regulation of IRS-1 plays a significant role in the tumor suppressor activity of miR145. J. Cell. Physiol. 220: 485–491, 2009. © 2009 Wiley-Liss, Inc. |
| Databáze: | OpenAIRE |
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