Rabbit Model of Physeal Injury for the Evaluation of Regenerative Medicine Approaches
| Autor: | Yangyi Yu, Nancy H. Miller, Asais Camila Uzcategui, Stephanie J. Bryant, Shane A Weatherford, Archish Muralidharan, Christopher B. Erickson, Virginia L. Ferguson, Karin A. Payne, Francisco Rodriguez-Fontan, Guangheng Li, Kevin Eckstein, Joseph R Fuchs |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
medicine.medical_specialty
0206 medical engineering Biomedical Engineering Salter-Harris Fractures Medicine (miscellaneous) Bioengineering 02 engineering and technology Regenerative Medicine Regenerative medicine 03 medical and health sciences New Zealand white rabbit Osteogenesis Deformity Animals Medicine Growth Plate Physis 030304 developmental biology 0303 health sciences biology business.industry Cartilage biology.organism_classification 020601 biomedical engineering Limb length Surgery Methods Articles Disease Models Animal Repair tissue medicine.anatomical_structure Rabbit model Rabbits medicine.symptom business |
| Zdroj: | Tissue Eng Part C Methods |
| ISSN: | 1937-3392 1937-3384 |
| DOI: | 10.1089/ten.tec.2019.0180 |
| Popis: | Physeal injuries can lead to bony repair tissue formation, known as a bony bar. This can result in growth arrest or angular deformity, which is devastating for children who have not yet reached their full height. Current clinical treatment involves resecting the bony bar and replacing it with a fat graft to prevent further bone formation and growth disturbance, but these treatments frequently fail to do so and require additional interventions. Novel treatments that could prevent bone formation but also regenerate the injured physeal cartilage and restore normal bone elongation are warranted. To test the efficacy of these treatments, animal models that emulate human physeal injury are necessary. The rabbit model of physeal injury quickly establishes a bony bar, which can then be resected to test new treatments. Although numerous rabbit models have been reported, they vary in terms of size and location of the injury, tools used to create the injury, and methods to assess the repair tissue, making comparisons between studies difficult. The study presented here provides a detailed method to create a rabbit model of proximal tibia physeal injury using a two-stage procedure. The first procedure involves unilateral removal of 25% of the physis in a 6-week-old New Zealand white rabbit. This consistently leads to a bony bar, significant limb length discrepancy, and angular deformity within 3 weeks. The second surgical procedure involves bony bar resection and treatment. In this study, we tested the implantation of a fat graft and a photopolymerizable hydrogel as a proof of concept that injectable materials could be delivered into this type of injury. At 8 weeks post-treatment, we measured limb length, tibial angle, and performed imaging and histology of the repair tissue. By providing a detailed, easy to reproduce methodology to perform the physeal injury and test novel treatments after bony bar resection, comparisons between studies can be made and facilitate translation of promising therapies toward clinical use. IMPACT STATEMENT: This study provides details to create a rabbit model of physeal injury that can facilitate comparisons between studies and test novel regenerative medicine approaches. Furthermore, this model mimics the human, clinical situation that requires a bony bar resection followed by treatment. In addition, identification of a suitable treatment can be seen in the correction of the growth deformity, allowing this model to facilitate the development of novel physeal cartilage regenerative medicine approaches. |
| Databáze: | OpenAIRE |
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