Detection of a novel herpesvirus from bats in the Philippines
| Autor: | Tetsuya Furuya, Edison Cosico, Tsutomu Omatsu, Hiroshi Shimoda, Tetsuya Mizutani, Mami Oba, Hiroomi Akashi, Joseph S. Masangkay, Eduardo Eres, Shigeru Kyuwa, Shumpei Mitomo, Yumi Une, Niña Quibod, Makoto Nagai, Sachiko Okazaki, Roberto Puentespina, Yukiko Sassa, Taisuke Kondo, Yukie Katayama, Ken Maeda, Satoshi Taniguchi, Kaori Sano, Yasuhiro Yoshikawa, Yuuki Hatta |
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| Rok vydání: | 2015 |
| Předmět: |
Sequence analysis
Philippines viruses Molecular Sequence Data Sequence Homology Biology medicine.disease_cause Polymerase Chain Reaction Genome Herpesviridae DNA sequencing law.invention Gammaherpesvirinae law Chiroptera Virology Genetics medicine Animals Cluster Analysis Molecular Biology Phylogeny Polymerase chain reaction Nucleic acid sequence Herpesviridae Infections Sequence Analysis DNA General Medicine Amplicon biology.organism_classification DNA Viral |
| Zdroj: | Virus Genes. 51:136-139 |
| ISSN: | 1572-994X 0920-8569 |
| DOI: | 10.1007/s11262-015-1197-6 |
| Popis: | Bats are natural hosts of many zoonotic viruses. Monitoring bat viruses is important to detect novel bat-borne infectious diseases. In this study, next generation sequencing techniques and conventional PCR were used to analyze intestine, lung, and blood clot samples collected from wild bats captured at three locations in Davao region, in the Philippines in 2012. Different viral genes belonging to the Retroviridae and Herpesviridae families were identified using next generation sequencing. The existence of herpesvirus in the samples was confirmed by PCR using herpesvirus consensus primers. The nucleotide sequences of the resulting PCR amplicons were 166-bp. Further phylogenetic analysis identified that the virus from which this nucleotide sequence was obtained belonged to the Gammaherpesvirinae subfamily. PCR using primers specific to the nucleotide sequence obtained revealed that the infection rate among the captured bats was 30 %. In this study, we present the partial genome of a novel gammaherpesvirus detected from wild bats. Our observations also indicate that this herpesvirus may be widely distributed in bat populations in Davao region. |
| Databáze: | OpenAIRE |
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