Differential gene regulation by Epstein-Barr virus type 1 and type 2 EBNA2
| Autor: | Gareth Brady, Michael Kracht, Oliver Dittrich-Breiholz, Walter Lucchesi, Paul J. Farrell, Rainer Russ |
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| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
Gene Expression Regulation
Viral Herpesvirus 4 Human Cell type viruses Immunology Apoptosis Viral transformation Biology medicine.disease_cause Microbiology Transformation and Oncogenesis Virus Cell Line Viral Matrix Proteins Viral Proteins immune system diseases hemic and lymphatic diseases Virology medicine Humans Lymphocytes Cell Proliferation Oligonucleotide Array Sequence Analysis Receptors CXCR Regulation of gene expression Cell growth virus diseases Transfection Cell Transformation Viral Epstein–Barr virus Molecular biology Cell Transformation Neoplastic Epstein-Barr Virus Nuclear Antigens Gene Expression Regulation Cell culture Insect Science |
| Popis: | A transfection assay with a lymphoblastoid cell line infected with Epstein-Barr virus was used to compare the abilities of type 1 and type 2 EBNA2 to sustain cell proliferation. The reduced proliferation in cells expressing type 2 EBNA2 correlated with loss of expression of some cell genes that are known to be targets of type 1 EBNA2. Microarray analysis of EBNA2 target genes identified a small number of genes that are more strongly induced by type 1 than by type 2 EBNA2, and one of these genes (CXCR7) was shown to be required for proliferation of lymphoblastoid cell lines. The Epstein-Barr virus LMP1 gene was also more strongly induced by type 1 EBNA2 than by type 2, but this effect was transient. Type 1 and type 2 EBNA2 were equally effective at arresting cell proliferation of Burkitt's lymphoma cell lines lacking Epstein-Barr virus and were also shown to cause apoptosis in these cells. The results indicate that differential gene regulation by Epstein-Barr virus type 1 and type 2 EBNA2 may be the basis for the much weaker B-cell transformation activity of type 2 Epstein-Barr virus strains compared to type 1 strains. |
| Databáze: | OpenAIRE |
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