Caprine pancreatic islet xenotransplantation into diabetic immunosuppressedBALB /c mice
| Autor: | Zeenathul Nazariah Allaudin, Abas Mazni Othman, Mohd-Azmi Mohd-Lila, Tengku Azmi Tengku Ibrahim, Homayoun Hani |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Blood Glucose
Male endocrine system medicine.medical_specialty endocrine system diseases type 1 diabetes medicine.medical_treatment Xenotransplantation Transplantation Heterologous Immunology Islets of Langerhans Transplantation Ischemia streptozotocin-injected mice Diabetes Mellitus Experimental BALB/c Islets of Langerhans Mice xenotransplantation Diabetes mellitus Internal medicine medicine Animals Mice Inbred BALB C Transplantation Type 1 diabetes Kidney geography geography.geographical_feature_category biology business.industry Goats Insulin Original Articles medicine.disease biology.organism_classification Islet caprine islets Endocrinology medicine.anatomical_structure business Immunosuppressive Agents |
| Zdroj: | Xenotransplantation |
| ISSN: | 1399-3089 0908-665X |
| DOI: | 10.1111/xen.12087 |
| Popis: | Background Type 1 diabetes mellitus is a devastating disease for which there is currently no cure, but only lifetime management. Islet xenotransplantation is a promising technique for the restoration of blood glucose control in patients with diabetes mellitus. The purpose of this study was to explore the potential use of caprine (goat) islet cells as xenogeneic grafts in the treatment for diabetes in a mouse model. Methods Caprine pancreases were harvested and transported to the laboratory under conditions optimized to prevent ischemia. Islets were isolated, purified, and tested for functionality. Caprine islets (2000 islet equivalent) were transplanted beneath the kidney capsules of diabetic BALB/c mice under thalidomide-induced immunosuppression. Blood glucose and insulin levels of grafted mice were evaluated by glucometer and enzyme-linked immunosorbent assay kit, respectively. The functionality and quality of caprine pancreatic islet grafts were assessed by intraperitoneal glucose tolerance tests. Results The viability of purified islet cells exceeded 90%. Recipient mice exhibited normoglycemia ( |
| Databáze: | OpenAIRE |
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