A rapid method for the isolation of functional thymus-derived murine lymphocytes
| Autor: | Leonore A. Herzenberg, M. H. Julius, Elizabeth Simpson |
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| Rok vydání: | 1973 |
| Předmět: |
Male
Erythrocytes T-Lymphocytes Immunology Population Cell Fluorescent Antibody Technique Immunoglobulins Hemolytic Plaque Technique Spleen Cell Separation Biology Lymphocyte Depletion Nitrophenols Mice medicine Animals Immunology and Allergy Cytotoxic T cell education Lymph node Incubation B-Lymphocytes Mice Inbred BALB C education.field_of_study Sheep Goats Immune Sera Macrophages Immunization Passive Cytotoxicity Tests Immunologic Molecular biology Nylons medicine.anatomical_structure biology.protein Female Immunization Glass Rabbits Antibody Filtration |
| Zdroj: | European Journal of Immunology. 3:645-649 |
| ISSN: | 1521-4141 0014-2980 |
| DOI: | 10.1002/eji.1830031011 |
| Popis: | A rapid method is described for effectively removing immunoglobulin-bearing cells from either primed or unprimed mouse spleen and lymph node cell suspensions. Incubation of cell suspensions in nylon wool columns for 45 min at 37 °C resulted in a 9 to 100-fold depletion of immunoglobulin-bearing cells and a complementary 1.5 to 2-fold enrichment of T cells in the column effluent populations. The effluent population, derived from passage of spleen cells through these columns, was virtually devoid of B precursor and memory cell activity, but contained all of the helper cell and cytotoxic effector cell precursor activity when compared to unfractionated spleen cells. |
| Databáze: | OpenAIRE |
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