Biological Tissue Imaging with a Position and Time Sensitive Pixelated Detector
| Autor: | Donald F. Smith, Jan Visser, Ivo Klinkert, Ron M. A. Heeren, Julia H. Jungmann, Luke MacAleese |
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| Rok vydání: | 2013 |
| Předmět: |
Male
Physics - Instrumentation and Detectors Materials science Microscope FOS: Physical sciences Field of view Mass spectrometry Sensitivity and Specificity Mass spectrometry imaging law.invention Mice Optics Microscopy Electron Transmission Structural Biology law Testis Image Processing Computer-Assisted Animals Image resolution Spectroscopy Pixel business.industry Histocytochemistry Detector Instrumentation and Detectors (physics.ins-det) Sample (graphics) Molecular Imaging Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization business Peptides |
| DOI: | 10.48550/arxiv.1305.5437 |
| Popis: | We demonstrate the capabilities of a highly parallel, active pixel detector for large-area, mass spectrometric imaging of biological tissue sections. A bare Timepix assembly (512x512 pixels) is combined with chevron microchannel plates on an ion microscope matrix-assisted laser desorption time-of-flight mass spectrometer (MALDI TOF-MS). The detector assembly registers position- and time-resolved images of multiple m/z species in every measurement frame. We prove the applicability of the detection system to bio-molecular mass spectrometry imaging on biologically relevant samples by mass-resolved images from Timepix measurements of a peptide-grid benchmark sample and mouse testis tissue slices. Mass-spectral and localization information of analytes at physiological concentrations are measured in MALDI-TOF-MS imaging experiments. We show a high spatial resolution (pixel size down to 740x740 nm2 on the sample surface) and a spatial resolving power of 6 {\mu}m with a microscope mode laser field of view of 100-335 {\mu}m. Automated, large-area imaging is demonstrated and the Timepix' potential for fast, large-area image acquisition is highlighted. |
| Databáze: | OpenAIRE |
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