Preparation de RNA Messager de Reticulocytes de Lapin par Ultracentrifugation en Rotor Zonal
| Autor: | Georges Huez, Arsène Burny, Eric Schram, Gérard Marbaix |
|---|---|
| Rok vydání: | 1967 |
| Předmět: |
Sulfates -- pharmacology
Messenger RNA Edetic Acid -- pharmacology Reticulocytes -- analysis Phosphorus Isotopes RNA Sciences bio-médicales et agricoles Ribosomes -- analysis Biology Isolation (microbiology) Biochemistry Molecular biology Solubility RNA Messenger -- analysis Centrifugation Density Gradient Methods RNA Transfer -- analysis Animals Sucrose gradient centrifugation Adsorption Rabbits |
| Zdroj: | European journal of biochemistry / FEBS, 1 (2 |
| ISSN: | 1432-1033 0014-2956 |
| DOI: | 10.1111/j.1432-1033.1967.tb00060.x |
| Popis: | Two methods are described for the rapid isolation of 0.6 mg to 1 mg of 9S RNA from rabbit reticulocytes. This RNA has properties expected for messenger RNA. In the first procedure, reticulocyte polyribosomes are dissolved in 0.5% (w/v) sodium dodecylsulfate and messenger RNA is separated by sucrose gradient centrifugation in a zonal rotor (B IV‐Spinco). In the second method, messenger RNA is detached from polyribosomes by EDTA (3x10−2M) treatment and separated from ribosomal sub‐particles by sucrose gradient centrifugation using the same rotor. In both cases, messenger RNA is then concentrated by adsorption on a non‐ionic cellulose column. Further purification may be obtained by sucrose gradient centrifugations using the rotor S. W. 25.2 (Spinco). Copyright © 1967, Wiley Blackwell. All rights reserved SCOPUS: ar.j info:eu-repo/semantics/published |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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