Kinome profiling of peripheral blood mononuclear cells collected prior to vaccination reveals biomarkers and potential mechanisms of vaccine unresponsiveness in pigs

Autor:	James Wilkinson, Sean Lipsit, Graham Plastow, Connor Denomy, Anthony Kusalik, Erin Scruten, Philip J. Griebel, Antonio Facciuolo, Scott Napper
Jazyk:	angličtina
Rok vydání:	2020
Předmět:	0301 basic medicine Male Transcription Genetic Swine medicine.medical_treatment lcsh:Medicine Predictive markers Peripheral blood mononuclear cell Article Herd immunity 03 medical and health sciences Interferon-gamma 0302 clinical medicine Immune system Medicine Animals Kinome 030212 general & internal medicine Phosphorylation lcsh:Science Inflammation Vaccines Multidisciplinary business.industry Vaccination lcsh:R Mycoplasma hyopneumoniae Pneumonia of Swine Mycoplasmal Antibodies Bacterial 030104 developmental biology Cytokine Animals Newborn Potential biomarkers Immunoglobulin G Immunology Cohort Bacterial Vaccines Leukocytes Mononuclear Cytokines Female lcsh:Q business Biomarkers Signal Transduction
Zdroj:	Scientific Reports, Vol 10, Iss 1, Pp 1-12 (2020) Scientific Reports
ISSN:	2045-2322
Popis:	Inter-individual variance in host immune responses following vaccination can result in failure to develop protective immunity leaving individuals at risk for infection in addition to compromising herd immunity. While developing more efficacious vaccines is one strategy to mitigate this problem, predicting vaccine responsiveness prior to vaccination could inform which individuals require adjunct disease management strategies. To identify biomarkers of vaccine responsiveness, a cohort of pigs (n = 120) were vaccinated and pigs representing the high (n = 6; 90th percentile) and low (n = 6; 10th percentile) responders based on vaccine-specific antibody responses following vaccination were further analyzed. Kinase-mediated phosphorylation events within peripheral blood mononuclear cells collected prior to vaccination identified 53 differentially phosphorylated peptides when comparing low responders with high responders. Functional enrichment analysis revealed pro-inflammatory cytokine signaling pathways as dysregulated, and this was further substantiated by detection of higher (p
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::81192398435697c757c6a3ea5af67506 http://link.springer.com/article/10.1038/s41598-020-68039-6 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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