Paclitaxel-loaded polymeric nanoparticles based on a-tocopheryl succinate for the treatment of head and neck squamous cell carcinoma: in vivo murine model

Autor: Joaquin Yanes-Diaz, Raquel Palao-Suay, Juan Riestra-Ayora, Carolina Sánchez-Rodríguez, Ana Martín-Hita, María Rosa Aguilar, Ricardo Sanz-Fernández
Přispěvatelé: Universidad Europea de Madrid, Centro de Investigación Biomédica en Red Bioingeniería, Biomateriales y Nanomedicina (España), Aguilar, María Rosa [0000-0001-7395-5754], Aguilar, María Rosa
Rok vydání: 2021
Předmět:
tumor xenograft mouse model
Tumor xenograft mouse model
Angiogenesis
Polymers
Chemistry
Pharmaceutical

Pharmaceutical Science
Apoptosis
02 engineering and technology
Polymeric nanoparticle
030226 pharmacology & pharmacy
Polyethylene Glycols
chemistry.chemical_compound
angiogenesis
Mice
Tratamiento médico
Hypopharynx carcinoma squamous cells
0302 clinical medicine
Tecnología médica
Drug Carriers
Neovascularization
Pathologic

Chemistry
General Medicine
Cáncer
021001 nanoscience & nanotechnology
Tumor Burden
tumor growth
Paclitaxel
Head and Neck Neoplasms
Female
0210 nano-technology
Research Article
Neoplasias de cabeza y cuello
Mice
Nude

RM1-950
03 medical and health sciences
In vivo
Cell Line
Tumor

medicine
Animals
Tumor growth
Cell Proliferation
Squamous Cell Carcinoma of Head and Neck
Cancer
hypopharynx carcinoma squamous cells
medicine.disease
Polymeric nanoparticles
Antineoplásicos
Head and neck squamous-cell carcinoma
Antineoplastic Agents
Phytogenic

Xenograft Model Antitumor Assays
Murine model
Cancer research
Nanoparticles
Therapeutics. Pharmacology
polymeric nanoparticle
Zdroj: ABACUS. Repositorio de Producción Científica
Universidad Europea (UEM)
Drug Delivery
article-version (VoR) Version of Record
Digital.CSIC. Repositorio Institucional del CSIC
instname
Drug Delivery, Vol 28, Iss 1, Pp 1376-1388 (2021)
Popis: The prognosis of patients with recurrent or metastatic head and neck squamous cell cancer (HNSCC) is generally poor. New treatments are required to supplement the current standard of care. Paclitaxel (PTX), an effective chemotherapeutic for HNSCC, has serious side effects. A polymeric nanocarrier system was developed for the delivery of PTX to improve HNSCC treatment. This study aimed to evaluate the antitumor efficacy of PTX-loaded polymeric nanoparticles based on α-TOS (PTX-NPs) administered by direct intratumoral injection into a Hypopharynx carcinoma squamous cells (FaDu) tumor xenograft mouse model. The nanocarrier system based on block copolymers of polyethylene glycol (PEG) and a methacrylic derivative of α-TOS was synthesized and PTX was loaded into the delivery system. Tumor volume was measured to evaluate the antitumor effect of the PTX-NPs. The relative mechanisms of apoptosis, cell proliferation, growth, angiogenesis, and oxidative and nitrosative stress were detected by Western blotting, fluorescent probes, and immunohistochemical analysis. The antitumor activity results showed that compared to free PTX, PTX-NPs exhibited much higher antitumor efficacy and apoptosis-inducing in a FaDu mouse xenograft model and demonstrated an improved safety profile. Ki-67, EGFR, and angiogenesis markers (Factor VIII, CD31, and CD34) expression were significantly lower in the PTX-NPs group compared with other groups (p
This work was supported by the Universidad Europea de Madrid under Grant 2016/UEM06 and 2017/UEM21; and Error! Hyperlink reference not valid., Biomateriales y Nanomedicina (CIBER-BBN) y la Fundación para la Excelencia y la Calidad en Oncología (ECO).
Databáze: OpenAIRE