Paclitaxel-loaded polymeric nanoparticles based on a-tocopheryl succinate for the treatment of head and neck squamous cell carcinoma: in vivo murine model
Autor: | Joaquin Yanes-Diaz, Raquel Palao-Suay, Juan Riestra-Ayora, Carolina Sánchez-Rodríguez, Ana Martín-Hita, María Rosa Aguilar, Ricardo Sanz-Fernández |
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Přispěvatelé: | Universidad Europea de Madrid, Centro de Investigación Biomédica en Red Bioingeniería, Biomateriales y Nanomedicina (España), Aguilar, María Rosa [0000-0001-7395-5754], Aguilar, María Rosa |
Rok vydání: | 2021 |
Předmět: |
tumor xenograft mouse model
Tumor xenograft mouse model Angiogenesis Polymers Chemistry Pharmaceutical Pharmaceutical Science Apoptosis 02 engineering and technology Polymeric nanoparticle 030226 pharmacology & pharmacy Polyethylene Glycols chemistry.chemical_compound angiogenesis Mice Tratamiento médico Hypopharynx carcinoma squamous cells 0302 clinical medicine Tecnología médica Drug Carriers Neovascularization Pathologic Chemistry General Medicine Cáncer 021001 nanoscience & nanotechnology Tumor Burden tumor growth Paclitaxel Head and Neck Neoplasms Female 0210 nano-technology Research Article Neoplasias de cabeza y cuello Mice Nude RM1-950 03 medical and health sciences In vivo Cell Line Tumor medicine Animals Tumor growth Cell Proliferation Squamous Cell Carcinoma of Head and Neck Cancer hypopharynx carcinoma squamous cells medicine.disease Polymeric nanoparticles Antineoplásicos Head and neck squamous-cell carcinoma Antineoplastic Agents Phytogenic Xenograft Model Antitumor Assays Murine model Cancer research Nanoparticles Therapeutics. Pharmacology polymeric nanoparticle |
Zdroj: | ABACUS. Repositorio de Producción Científica Universidad Europea (UEM) Drug Delivery article-version (VoR) Version of Record Digital.CSIC. Repositorio Institucional del CSIC instname Drug Delivery, Vol 28, Iss 1, Pp 1376-1388 (2021) |
Popis: | The prognosis of patients with recurrent or metastatic head and neck squamous cell cancer (HNSCC) is generally poor. New treatments are required to supplement the current standard of care. Paclitaxel (PTX), an effective chemotherapeutic for HNSCC, has serious side effects. A polymeric nanocarrier system was developed for the delivery of PTX to improve HNSCC treatment. This study aimed to evaluate the antitumor efficacy of PTX-loaded polymeric nanoparticles based on α-TOS (PTX-NPs) administered by direct intratumoral injection into a Hypopharynx carcinoma squamous cells (FaDu) tumor xenograft mouse model. The nanocarrier system based on block copolymers of polyethylene glycol (PEG) and a methacrylic derivative of α-TOS was synthesized and PTX was loaded into the delivery system. Tumor volume was measured to evaluate the antitumor effect of the PTX-NPs. The relative mechanisms of apoptosis, cell proliferation, growth, angiogenesis, and oxidative and nitrosative stress were detected by Western blotting, fluorescent probes, and immunohistochemical analysis. The antitumor activity results showed that compared to free PTX, PTX-NPs exhibited much higher antitumor efficacy and apoptosis-inducing in a FaDu mouse xenograft model and demonstrated an improved safety profile. Ki-67, EGFR, and angiogenesis markers (Factor VIII, CD31, and CD34) expression were significantly lower in the PTX-NPs group compared with other groups (p This work was supported by the Universidad Europea de Madrid under Grant 2016/UEM06 and 2017/UEM21; and Error! Hyperlink reference not valid., Biomateriales y Nanomedicina (CIBER-BBN) y la Fundación para la Excelencia y la Calidad en Oncología (ECO). |
Databáze: | OpenAIRE |
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