Effects of Acute Exercise on Mitochondrial Function, Dynamics, and Mitophagy in Rat Cardiac and Skeletal Muscles
| Autor: | Jun-Won Heo, Dae Yun Seo, Ju-Hee Kang, Mi-Hyun No, Hyo-Bum Kwak, Su-Jeen Jung, Jin Han, Su-Zi Yoo, Joon-Hee Kim, Dong-Ho Park |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
FIS1
medicine.medical_specialty Urology 030232 urology & nephrology MFN2 Skeletal muscle PINK1 lcsh:RC870-923 03 medical and health sciences 0302 clinical medicine Internal medicine Mitophagy medicine Soleus muscle 030219 obstetrics & reproductive medicine business.industry Heart Acute exercise lcsh:Diseases of the genitourinary system. Urology Dynamics Endocrinology medicine.anatomical_structure Neurology mitochondrial fusion Original Article Mitochondrial fission Neurology (clinical) Mitochondrial function business |
| Zdroj: | International Neurourology Journal, Vol 23, Iss Suppl 1, Pp S22-31 (2019) International Neurourology Journal |
| ISSN: | 2093-6931 2093-4777 |
| Popis: | Purpose This study aimed to investigate the effects of single-bout exercise on mitochondrial function, dynamics (fusion, fission), and mitophagy in cardiac and skeletal muscles. Methods Fischer 344 rats (4 months old) were randomly divided into the control (CON) or acute exercise (EX) group (n=10 each). The rats performed a single bout of treadmill exercise for 60 minutes. Mitochondrial function (e.g., O2 respiration, H2O2 emission, Ca2+ retention capacity), mitochondrial fusion (e.g., Mfn1, Mfn2, Opa1), mitochondrial fission (e.g., Drp1, Fis1), and mitophagy (e.g., Parkin, Pink1, LC3II, Bnip3) were measured in permeabilized cardiac (e.g., left ventricle) and skeletal (e.g., soleus, white gastrocnemius) muscles. Results Mitochondrial O2 respiration and Ca2+ retention capacity were significantly increased in all tissues of the EX group compared with the CON group. Mitochondrial H2O2 emissions showed tissue-specific results; the emissions showed no significant differences in the left ventricle or soleus (type I fibers) but was significantly increased in the white gastrocnemius (type II fibers) after acute exercise. Mitochondrial fusion and fission were not altered in any tissues of the EX group. Mitophagy showed tissue-specific differences: It was not changed in the left ventricle or white gastrocnemius, whereas Parkin and LC3II were significantly elevated in the soleus muscle. Conclusion A single bout of aerobic exercise may improve mitochondrial function (e.g., O2 respiration and Ca2+ retention capacity) in the heart and skeletal muscles without changes in mitochondrial dynamics or mitophagy. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|