The use ofStreptococcus zymogenes for estimating tryptophan and methionine bioavailability in 17 foods
| Autor: | F. E. McDonough, C. E. Bodwell, Patricia Wells, Anthony Hitchens |
|---|---|
| Rok vydání: | 1989 |
| Předmět: |
chemistry.chemical_classification
Methionine Tryptophan Streptococcus Pronase In Vitro Techniques Biology Defatting Bioavailability chemistry.chemical_compound Enzyme Biochemistry chemistry Chemistry (miscellaneous) Biological Assay Composition (visual arts) Nutritive Value Protein quality Food Science |
| Zdroj: | Plant Foods for Human Nutrition. 39:121-127 |
| ISSN: | 1573-9104 0921-9668 |
| DOI: | 10.1007/bf01092408 |
| Popis: | As part of a cooperative study assessing amino acid bioavailability and/or protein quality, the provisional method of Boyne et al. (Brit J Nutr 21: 181-206) was used to assay 17 protein sources for methionine and tryptophan availability with S. zymogenes. Pronase was used as the predigesting enzyme. Product composition was found to affect reproducibility. The microbial assay results correlated positively with results from rat growth studies on the same foods (p = 0.05), and were generally accurate in identifying products of lower protein quality. Defatting four high-fat products increased microbial values in the methionine assay, but only the chicken franks and the sausage values in the tryptophan assay. Heating non-fat milk increased methionine values slightly. Low values for rolled oats were further reduced by finer grinding. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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