Serotyping of Cryptococcus neoformans by dot enzyme assay

Autor: T Belay, T R Kozel, E B O'Neill, Robert Cherniak
Rok vydání: 1996
Předmět:
Zdroj: Journal of clinical microbiology. 34(2)
ISSN: 0095-1137
Popis: A method is described for the serotyping of Cryptococcus neoformans based on direct analysis of culture supernatants for the major type-specific capsular antigen, glucuronoxylomannan. Factor sera prepared by absorption of polyclonal rabbit antisera (Iatron Laboratories, Inc., Tokyo, Japan) or selected anti-C. neoformans monoclonal antibodies were used in a dot enzyme assay to detect the presence of antigen.
Databáze: OpenAIRE