Changes in the blood fatty-acid profile associated with oxidative-antioxidant disturbances in coronary atherosclerosis
| Autor: | E.M. Stakhneva, E. I. Chernyak, Yuliya I. Ragino, V. Shramko, Y. Polonskaya, Elena Shakhtshneider, Liliia V. Shcherbakova, Sergey V. Morozov, Alexander M. Chernyavskiy |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
medicine.medical_specialty
Antioxidant medicine.medical_treatment Oxidative phosphorylation 030204 cardiovascular system & hematology Phospholipase atherosclerotic plaque medicine.disease_cause lcsh:Biochemistry 03 medical and health sciences 0302 clinical medicine Phospholipase A2 lipid Internal medicine medicine oxidative stress lcsh:QD415-436 030212 general & internal medicine Coronary atherosclerosis chemistry.chemical_classification Original Paper biology business.industry Fatty acid Endocrinology chemistry biology.protein fatty acid atherosclerosis business Oxidative stress lipoprotein-associated phospholipase 2 Polyunsaturated fatty acid |
| Zdroj: | Journal of Medical Biochemistry, Vol 39, Iss 1, Pp 46-53 (2020) J Med Biochem |
| ISSN: | 1452-8266 1452-8258 |
| Popis: | The objective of this work was to study the profile of fatty acids and to search for associations of fatty acids with oxidative-antioxidant parameters and an oxidative-inflammatory biomarker (lipoprotein-associated phospholipase A2) in men with coronary atherosclerosis and coronary heart disease.Analysis of 20 fatty acids was performed in 60 men with angiographically confirmed coronary atherosclerosis and coronary heart disease and in a control group of men without coronary heart disease. Serum fatty-acid content was evaluated by high-performance gas-liquid chromatography. The blood levels of oxidative stress, total antioxidative defence, and lipoprotein-associated phospholipase 2 were analyzed.In the group of men with coronary atherosclerosis the levels of myristic and palmitic fatty acids were higher by 59% and 22%, respectively. An increase in the weight percentage of monounsaturated fatty acids was noted, such as palmitoleic, oleic, and octadecenic. Significantly lower levels of polyunsaturated fatty acids, such as linolic, eicosadienoic, eicosatrienoic, arachidonic, eicosapentaenoic, glinolenic, docosapentaenoic, and docosahexaenoic were detected in the group with coronary atherosclerosis. The lipoprotein-associated phospholipase A2 level was higher by 48%. Oxidative stress was higher by 17%, and the total antioxidant defence in serum was lower by 45%. We found correlations between fatty acids and oxidative-antioxidative alterations. The relative risk of vulnerable atherosclerotic plaques correlated with increased levels of palmitic, stearic, oleic, and linolic fatty acids.Significant alterations in the profile of fatty acids are associated with oxidative-antioxidative alterations and are accompanied by an increase in free-radical formation, which can probably serve as a risk factor of atherosclerosis.Cilj ovog rada bio je da se ispita profil masnih kiselina i da se istraže veze masnih kiselina sa oksidativno-antioksidativnim parametrima i oksidativno-inflamatornim biomarkerom (lipoprotein - udružena fosfolipaza A2) kod muškaraca sa koronarnom aterosklerozom i koronarnom bolešću srca.Sprovedena je analiza 20 masnih kiselina kod 60 muškaraca sa angiografski potvrđenom koronarnom aterosklerozom i koronarnom bolešću srca i kod kontrolne grupe muškaraca bez koronarne bolesti srca. Sadržaj masnih kiselina u serumu je procenjen gasnom-tečnom hromatografijom visokih performansi. Analizirani su nivoi oksidativnog stresa u krvi, ukupne antioksidativne odbrane i lipoproteinasocirane fosfolipaze A2.U grupi muškaraca sa koronarnom aterosklerozom, nivo mirističkih i palmitinskih masnih kiselina bio je veći za 59% i 22%, respektivno. Uočen je porast u težinskom procentu mononezasićenih masnih kiselina, kao što su palmitoleinska, oleinska i oktadeceinska. U grupi sa koronarnom arterosklerozom otkriveni su znatno niži nivoi polinezasićenih masnih kiselina, kao što su linolna, eikosadie noična, eikosatrienoična, arahidonska, eikos apentaenska, g-linolenska, dokosapentaenoična i dokosaheksaenoična. Nivo lipoprotein-asocirane fosfolipaze A2 bio je viši za 48%. Oksidativni stres je bio veći za 17%, a ukupna antioksidativna odbrana u serumu bila je niža za 45%. Pronašli smo korelacije između masnih kiselina i oksidativno-antioksidativnih promena. Relativni rizik od nestabilnih aterosklerotskih plakova korelira sa povećanim nivoima palmitinske, stearinske, oleinske i linolne masne kiseline.Značajne promene u profilu masnih kiselina povezane su sa oksidativno-antioksidativnim promenama i praćene su porastom formiranja slobodnih radikala, što verovatno može da posluži kao faktor rizika za aterosklerozu. |
| Databáze: | OpenAIRE |
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