Kinetics of cytomegalovirus and Epstein-Barr virus DNA in whole blood and plasma of kidney transplant recipients: Implications on management strategies

Autor: Tiziana Lazzarotto, Angela Chiereghin, Antonio Piralla, Dino Gibertoni, Giulia Piccirilli, Gabriele Turello, Giulia Campanini, Liliana Gabrielli, Cristina Costa, Giorgia Comai, Gaetano La Manna, Luigi Biancone, Teresa Rampino, Marilena Gregorini, Francesca Sidoti, Gabriele Bianco, Maria Vittoria Mauro, Francesca Greco, Rossana Cavallo, Fausto Baldanti, AMCLI-GLaIT
Přispěvatelé: Lazzarotto T, Chiereghin A, Piralla A, Gibertoni D, Piccirilli G, Turello G, Campanini G, Gabrielli L, Costa C, Comai G, La Manna G, Biancone L, Rampino T, Gregorini M, Sidoti F, Bianco G, Mauro MV, Greco F, Cavallo R, Baldanti F, AMCLI-GLaIT.
Rok vydání: 2020
Předmět:
0301 basic medicine
Human cytomegalovirus
Herpesvirus 4
Human
Physiology
Cancer Treatment
Cytomegalovirus
030230 surgery
Pathology and Laboratory Medicine
medicine.disease_cause
Gastroenterology
Cohort Studies
0302 clinical medicine
Blood plasma
Medicine and Health Sciences
Public and Occupational Health
Viral
Cytomegalovirus
Epstein-Barr virus
viral DNA in whole blood
viral DNA in plasma
kidney transplant recipients
Kidney transplantation
Virus Testing
Whole blood
Multidisciplinary
virus diseases
Viral Load
Vaccination and Immunization
Body Fluids
Blood
Oncology
Medical Microbiology
Viral Pathogens
Viruses
Medicine
Human Cytomegalovirus
Anatomy
Pathogens
Viral load
Immunosuppressive Agents
Research Article
Human
Adult
Herpesviruses
medicine.medical_specialty
Science
Immunology
Antiviral Agents
DNA
Viral
Humans
Kinetics
Retrospective Studies
Kidney Transplantation
Congenital cytomegalovirus infection
Microbiology
Blood Plasma
Virus
03 medical and health sciences
Antiviral Therapy
Antibody Therapy
Diagnostic Medicine
Virology
Internal medicine
medicine
Epstein-Barr virus
Microbial Pathogens
business.industry
Organisms
Herpesvirus 4
Biology and Life Sciences
DNA
medicine.disease
Epstein–Barr virus
030104 developmental biology
Clinical Immunology
Preventive Medicine
Clinical Medicine
DNA viruses
business
Viral Transmission and Infection
Zdroj: PLoS ONE
PLoS ONE, Vol 15, Iss 8, p e0238062 (2020)
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0238062
Popis: This retrospective multicenter cohort study investigated the kinetics (ascending and descending phases) of cytomegalovirus (CMV) and Epstein-Barr virus (EBV)-DNA in whole blood (WB) and plasma samples collected from adult kidney transplant (KT) recipients. CMV-DNA kinetics according to antiviral therapy were investigated. Three hundred twenty-eight paired samples from 42 episodes of CMV infection and 157 paired samples from 26 episodes of EBV infection were analyzed by a single commercial molecular method approved by regulatory agencies for both matrices. CMV-DNAemia followed different kinetics in WB and plasma. In the descending phase of infection, a slower decay of viral load and a higher percentage of CMV-DNA positive samples were observed in plasma versus WB. In the 72.4% of patients receiving antiviral therapy, monitoring with plasma CMV-DNAemia versus WB CMV-DNAemia could delay treatment interruption by 7-14 days. Discontinuation of therapy based on WB monitoring did not result in relapsed infection in any patients. Highly different EBV-DNA kinetics in WB and plasma were observed due to lower positivity in plasma; EBV positive samples with a quantitative result in both blood compartments were observed in only 11.5% of cases. Our results emphasize the potential role of WB as specimen type for post-KT surveillance of both infections for disease prevention and management.
Databáze: OpenAIRE
Externí odkaz: