Determining the refractive index of human hemoglobin solutions by Kramers-Kronig relations with an improved absorption model
| Autor: | Jörg Neukammer, Hermann Groß, Jonas Gienger, Markus Bär |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Materials science Kramers–Kronig relations Absorption spectroscopy Materials Science (miscellaneous) Analytical chemistry FOS: Physical sciences medicine.disease_cause 01 natural sciences Industrial and Manufacturing Engineering Light scattering 010309 optics Absorbance 03 medical and health sciences Optics 0103 physical sciences medicine Physics - Biological Physics Business and International Management Absorption (electromagnetic radiation) business.industry Physics - Medical Physics 030104 developmental biology Biological Physics (physics.bio-ph) Attenuation coefficient Medical Physics (physics.med-ph) business Refractive index Ultraviolet Optics (physics.optics) Physics - Optics |
| Zdroj: | Applied optics. 55(31) |
| ISSN: | 1539-4522 |
| Popis: | The real part of the refractive index (RI) of aqueous solutions of human hemoglobin is computed from their absorption spectra in the wavelength range $250\,{\rm nm} - 1100\,{\rm nm}$ using the Kramers-Kronig (KK) relations and the corresponding uncertainty analysis is provided. The strong ultraviolet (UV) and infrared absorbance of the water outside this spectral range were taken into account in a previous study employing KK relations. We improve these results by including the concentration dependence of the water absorbance as well as by modeling the deep UV absorbance of hemoglobin's peptide backbone. The two free parameters of the model for the deep UV absorbance are fixed by a global fit. \copyright{} 2016 Optical Society of America. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modifications of the content of this paper are prohibited |
| Databáze: | OpenAIRE |
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