TGF-beta 1 production in radiation nephropathy: role of angiotensin II
| Autor: | P K, Datta, J E, Moulder, B L, Fish, E P, Cohen, E A, Lianos |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Radiation Nephropathy
Angiotensin receptor medicine.medical_specialty medicine.drug_class Kidney Glomerulus Tetrazoles Nephropathy Angiotensin Receptor Antagonists Transforming Growth Factor beta Internal medicine medicine Animals Radiology Nuclear Medicine and imaging Blood urea nitrogen TGF beta 1 Antihypertensive Agents Kidney Radiological and Ultrasound Technology biology Chemistry Angiotensin II Imidazoles Rats Inbred Strains medicine.disease Receptor antagonist Rats Radiation Injuries Experimental medicine.anatomical_structure Endocrinology biology.protein Female Kidney Diseases |
| Zdroj: | International journal of radiation biology. 75(4) |
| ISSN: | 0955-3002 |
| Popis: | Angiotensin II receptor antagonists are effective in the prophylaxis of radiation nephropathy. Studies were designed to determine whether TGF-beta 1, a fibrogenic cytokine, plays a role in mediating the protective effect of AII antagonism. These studies explored the time-course of glomerular TGF-beta 1 production in the irradiated kidney, and whether AII mediates TGF-beta 1 production in glomeruli isolated from irradiated rats.Rats received 20 Gy of bilateral renal irradiation in five fractions and were randomized to receive an AII type 1 receptor antagonist (L-158,809) at 20mg/l in their drinking water, or no treatment. Drug therapy began 9 days prior to irradiation and continued for the duration of the study.Analysis of renal function showed a significant increase in urinary proteinuria and blood urea nitrogen by 37 days and 63 days after irradiation, respectively. Estimation of glomerular TGF-beta1 levels by quantitative sandwich enzyme immunoassay technique revealed a significant increase in latent but not active TGF-beta 1 levels at 50 days and 63 days after irradiation. In animals treated with the AT1 receptor antagonist, there was a complete elimination in the rise of TGF-beta 1.These studies demonstrate that glomerular TGF-beta 1 production is elevated in the course of radiation nephropathy, and that AII mediates this induction of TGF-beta 1. |
| Databáze: | OpenAIRE |
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