Evidence for a role of Arabidopsis CDT1 proteins in gametophyte development and maintenance of genome integrity

Autor: Sophie Blanchet, Séverine Domenichini, Mickael Bourge, Lieven De Veylder, Moussa Benhamed, Eveline Van De Slijke, Catherine Bergounioux, Geert De Jaeger, Cécile Raynaud
Přispěvatelé: Institut de biotechnologie des plantes (IBP), Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS), Institut des sciences du végétal (ISV), Centre National de la Recherche Scientifique (CNRS), Center for Plant Systems Biology (PSB Center), Vlaams Instituut voor Biotechnologie [Ghent, Belgique] (VIB)
Jazyk: angličtina
Rok vydání: 2012
Předmět:
0106 biological sciences
Models
Molecular

MESH: Genome
Plant

DNA Repair
Arabidopsis
MESH: Plant Roots
TANDEM AFFINITY PURIFICATION
Eukaryotic DNA replication
Cell Cycle Proteins
Plant Science
Pre-replication complex
MESH: Germ Cells
Plant

01 natural sciences
Plant Roots
MESH: Down-Regulation
Gene Expression Regulation
Plant

MESH: Arabidopsis
Research Articles
Genetics
MESH: DNA Repair
0303 health sciences
MESH: Genomic Instability
PLANT DEVELOPMENT
PROLIFERATION
Endoreduplication
Plants
Genetically Modified

FEMALE GAMETOPHYTE
MESH: Plant Leaves
Phenotype
S-PHASE
embryonic structures
Pollen
RNA Interference
Genome
Plant

MESH: Models
Molecular

DNA re-replication
DNA repair
MESH: RNA Interference
Down-Regulation
MESH: Arabidopsis Proteins
Biology
MESH: Phenotype
MESH: Two-Hybrid System Techniques
Genomic Instability
03 medical and health sciences
MESH: Cell Cycle Proteins
Control of chromosome duplication
Two-Hybrid System Techniques
[SDV.BV]Life Sciences [q-bio]/Vegetal Biology
CELL-CYCLE
MESH: Gene Expression Regulation
Plant

Replication protein A
030304 developmental biology
MESH: DNA Damage
COMPLEX
THALIANA
Arabidopsis Proteins
DNA replication
Biology and Life Sciences
Cell Biology
MESH: Gamma Rays
Plant Leaves
Mutagenesis
Insertional

DNA-POLYMERASE-EPSILON
MESH: Mutagenesis
Insertional

MESH: Plants
Genetically Modified

Gamma Rays
MESH: Pollen
REPLICATION
Origin recognition complex
Germ Cells
Plant

010606 plant biology & botany
DNA Damage
MESH: Endoreduplication
Zdroj: PLANT CELL
The Plant cell
The Plant cell, American Society of Plant Biologists (ASPB), 2012, 24 (7), pp.2779-91. ⟨10.1105/tpc.112.100156⟩
ISSN: 1040-4651
1532-298X
DOI: 10.1105/tpc.112.100156⟩
Popis: International audience; Meristems retain the ability to divide throughout the life cycle of plants, which can last for over 1000 years in some species. Furthermore, the germline is not laid down early during embryogenesis but originates from the meristematic cells relatively late during development. Thus, accurate cell cycle regulation is of utmost importance to avoid the accumulation of mutations during vegetative growth and reproduction. The Arabidopsis thaliana genome encodes two homologs of the replication licensing factor CDC10 Target1 (CDT1), and overexpression of CDT1a stimulates DNA replication. Here, we have investigated the respective functions of Arabidopsis CDT1a and CDT1b. We show that CDT1 proteins have partially redundant functions during gametophyte development and are required for the maintenance of genome integrity. Furthermore, CDT1-RNAi plants show endogenous DNA stress, are more tolerant than the wild type to DNA-damaging agents, and show constitutive induction of genes involved in DNA repair. This DNA stress response may be a direct consequence of reduced CDT1 accumulation on DNA repair or may relate to the ability of CDT1 proteins to form complexes with DNA polymerase ε, which functions in DNA replication and in DNA stress checkpoint activation. Taken together, our results provide evidence for a crucial role of Arabidopsis CDT1 proteins in genome stability.
Databáze: OpenAIRE