Evidence for a role of Arabidopsis CDT1 proteins in gametophyte development and maintenance of genome integrity
Autor: | Sophie Blanchet, Séverine Domenichini, Mickael Bourge, Lieven De Veylder, Moussa Benhamed, Eveline Van De Slijke, Catherine Bergounioux, Geert De Jaeger, Cécile Raynaud |
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Přispěvatelé: | Institut de biotechnologie des plantes (IBP), Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS), Institut des sciences du végétal (ISV), Centre National de la Recherche Scientifique (CNRS), Center for Plant Systems Biology (PSB Center), Vlaams Instituut voor Biotechnologie [Ghent, Belgique] (VIB) |
Jazyk: | angličtina |
Rok vydání: | 2012 |
Předmět: |
0106 biological sciences
Models Molecular MESH: Genome Plant DNA Repair Arabidopsis MESH: Plant Roots TANDEM AFFINITY PURIFICATION Eukaryotic DNA replication Cell Cycle Proteins Plant Science Pre-replication complex MESH: Germ Cells Plant 01 natural sciences Plant Roots MESH: Down-Regulation Gene Expression Regulation Plant MESH: Arabidopsis Research Articles Genetics MESH: DNA Repair 0303 health sciences MESH: Genomic Instability PLANT DEVELOPMENT PROLIFERATION Endoreduplication Plants Genetically Modified FEMALE GAMETOPHYTE MESH: Plant Leaves Phenotype S-PHASE embryonic structures Pollen RNA Interference Genome Plant MESH: Models Molecular DNA re-replication DNA repair MESH: RNA Interference Down-Regulation MESH: Arabidopsis Proteins Biology MESH: Phenotype MESH: Two-Hybrid System Techniques Genomic Instability 03 medical and health sciences MESH: Cell Cycle Proteins Control of chromosome duplication Two-Hybrid System Techniques [SDV.BV]Life Sciences [q-bio]/Vegetal Biology CELL-CYCLE MESH: Gene Expression Regulation Plant Replication protein A 030304 developmental biology MESH: DNA Damage COMPLEX THALIANA Arabidopsis Proteins DNA replication Biology and Life Sciences Cell Biology MESH: Gamma Rays Plant Leaves Mutagenesis Insertional DNA-POLYMERASE-EPSILON MESH: Mutagenesis Insertional MESH: Plants Genetically Modified Gamma Rays MESH: Pollen REPLICATION Origin recognition complex Germ Cells Plant 010606 plant biology & botany DNA Damage MESH: Endoreduplication |
Zdroj: | PLANT CELL The Plant cell The Plant cell, American Society of Plant Biologists (ASPB), 2012, 24 (7), pp.2779-91. ⟨10.1105/tpc.112.100156⟩ |
ISSN: | 1040-4651 1532-298X |
DOI: | 10.1105/tpc.112.100156⟩ |
Popis: | International audience; Meristems retain the ability to divide throughout the life cycle of plants, which can last for over 1000 years in some species. Furthermore, the germline is not laid down early during embryogenesis but originates from the meristematic cells relatively late during development. Thus, accurate cell cycle regulation is of utmost importance to avoid the accumulation of mutations during vegetative growth and reproduction. The Arabidopsis thaliana genome encodes two homologs of the replication licensing factor CDC10 Target1 (CDT1), and overexpression of CDT1a stimulates DNA replication. Here, we have investigated the respective functions of Arabidopsis CDT1a and CDT1b. We show that CDT1 proteins have partially redundant functions during gametophyte development and are required for the maintenance of genome integrity. Furthermore, CDT1-RNAi plants show endogenous DNA stress, are more tolerant than the wild type to DNA-damaging agents, and show constitutive induction of genes involved in DNA repair. This DNA stress response may be a direct consequence of reduced CDT1 accumulation on DNA repair or may relate to the ability of CDT1 proteins to form complexes with DNA polymerase ε, which functions in DNA replication and in DNA stress checkpoint activation. Taken together, our results provide evidence for a crucial role of Arabidopsis CDT1 proteins in genome stability. |
Databáze: | OpenAIRE |
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