The two main forms of histiocytic sarcoma in the predisposed Flatcoated retriever dog display variation in gene expression
| Autor: | Boerkamp, Kim M, van Steenbeek, Frank G, Penning, Louis C, Groot Koerkamp, Marian J A, van Leenen, Dik, Vos-Loohuis, Manon, Grinwis, Guy C M, Rutteman, Gerard R, Tissue Repair, Sub Oncologie/Cytologie, CSCA AVM, Onderzoek, Universitair Veterinair Diagnostisch Lab, CSCA TR1, Biochemisch laboratorium, Veterinair Pathologisch Diagnostisch Cnt, PB AVM, Dep Pathobiologie, CSCA TR2 |
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| Přispěvatelé: | Tissue Repair, Sub Oncologie/Cytologie, CSCA AVM, Onderzoek, Universitair Veterinair Diagnostisch Lab, CSCA TR1, Biochemisch laboratorium, Veterinair Pathologisch Diagnostisch Cnt, PB AVM, Dep Pathobiologie, CSCA TR2 |
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Veterinary Medicine
Pathology medicine.medical_specialty Microarray 040301 veterinary sciences lcsh:Medicine Histiocytic sarcoma Biology Real-Time Polymerase Chain Reaction medicine.disease_cause 0403 veterinary science 03 medical and health sciences Dogs Basic Cancer Research Gene expression Genetics Cancer Genetics Medicine and Health Sciences medicine Animals Genetic Predisposition to Disease Dog Diseases lcsh:Science Histiocyte DNA Primers 030304 developmental biology 0303 health sciences Multidisciplinary Base Sequence Gene Expression Profiling lcsh:R Biology and Life Sciences 04 agricultural and veterinary sciences medicine.disease Gene expression profiling Real-time polymerase chain reaction Veterinary Diseases Oncology Veterinary Oncology Veterinary Science lcsh:Q Histiocytic Sarcoma DNA microarray Carcinogenesis Animal Genetics Veterinary Pathology Research Article |
| Zdroj: | PLoS ONE, Vol 9, Iss 6, p e98258 (2014) PLoS ONE PLoS One, 9(6). Public Library of Science |
| ISSN: | 1932-6203 |
| Popis: | Examination of gene functions in specific tumor types improves insight in tumorigenesis and helps design better treatments. Due to the rarity of histiocytic/dendritic cell sarcoma in humans, it is difficult to accrue such knowledge. Therefore, comparative research of these cancers in predisposed dog breeds, such as the Flatcoated retriever, can be of value. Histiocytic sarcoma in the dog can be grouped into a soft tissue- and visceral form. The soft tissue form at first is localized, while the visceral form progresses more quickly to a terminal state, which might be related to variations in gene expression. Microarray analyses were performed on fresh-frozen tissue from Flatcoated retrievers with either soft tissue- or visceral histiocytic sarcoma. Expression differences of ten most significantly differentially expressed genes were validated with quantitative real-time PCR (q PCR) analyses. Q PCR analyses confirmed the significantly aberrant expression of three of the selected genes: C6 was up-regulated; CLEC12A and CCL5 were down-regulated in the visceral histiocytic sarcoma compared to the soft tissue form. The findings of our study indicate that these two forms of histiocytic sarcoma in the dog display a variation in gene expression and warrant analysis of functional changes in the expression of those genes in these rare sarcomas in man. |
| Databáze: | OpenAIRE |
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