Usefulness of à la carte antigens for bird fancier’s lung serodiagnosis: total dropping extract and/or dropping’s microflora antigens

Autor: Sandrine Roussel, Audrey Laboissière, Benoît Valot, Jean-Charles Dalphin, Adeline Rouzet, Paul De Vuyst, Gabriel Reboux, Laurence Millon
Přispěvatelé: Laboratoire Chrono-environnement ( LCE ), Université Bourgogne Franche-Comté ( UBFC ) -Centre National de la Recherche Scientifique ( CNRS ) -Université de Franche-Comté ( UFC ), Service de parasitologie et mycologie [CHU de Besançon], Centre Hospitalier Régional Universitaire [Besançon] ( CHRU Besançon ), Service de Pneumologie, Hôpital Jean-Minjoz, Centre Hospitalier Universitaire de Besançon, Université de Franche-Comté, Besançon, France., Erasme Hospital, Brussels, Hôpital Jean Minjoz, Laboratoire Chrono-environnement - CNRS - UBFC (UMR 6249) (LCE), Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Centre National de la Recherche Scientifique (CNRS), Service de parasitologie et mycologie [CHRU de Besançon], Centre Hospitalier Régional Universitaire de Besançon (CHRU Besançon), Service de Pneumologie, oncologie thoracique et allergologie respiratoire [CHRU Besançon], Centre Hospitalier Régional Universitaire de Besançon (CHRU Besançon)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), Hôpital Erasme [Bruxelles] (ULB), Faculté de Médecine [Bruxelles] (ULB), Université libre de Bruxelles (ULB)-Université libre de Bruxelles (ULB)
Rok vydání: 2017
Předmět:
diagnosis
MESH : Antigens
serology
Serology
Feces
0302 clinical medicine
Bird fancier's lung
antigens
Flora (microbiology)
MESH : Bird Fancier's Lung
MESH: Animals
030212 general & internal medicine
microorganisms
MESH: Bird Fancier's Lung
MESH : Allergens
Immunoassay
biology
MESH: Feces
General Medicine
Enterobacter
MESH : Immunoassay
MESH : Serologic Tests
3. Good health
medicine.anatomical_structure
MESH: Birds
MESH: Antigens
Antibody
medicine.symptom
MESH: Immunoassay
MESH: Fungi
Microbiology (medical)
MESH: Allergens
droppings
Microbiology
Asymptomatic
[ SDV.EE.SANT ] Life Sciences [q-bio]/Ecology
environment/Health
Birds
03 medical and health sciences
Antigen
medicine
Animals
Humans
Serologic Tests
MESH : Bacteria
MESH : Birds
MESH : Fungi
bird fancier's lung
[SDV.EE.SANT]Life Sciences [q-bio]/Ecology
environment/Health
MESH: Humans
Lung
Bacteria
MESH: Serologic Tests
MESH : Humans
Fungi
MESH : Feces
Allergens
medicine.disease
biology.organism_classification
MESH: Bacteria
030228 respiratory system
Immunology
biology.protein
MESH : Animals
[ SDV.MHEP.PSR ] Life Sciences [q-bio]/Human health and pathology/Pulmonology and respiratory tract
Zdroj: Journal of Medical Microbiology
Journal of Medical Microbiology, Society for General Microbiology, 2017, 66 (10), pp.1467-1470. 〈10.1099/jmm.0.000586〉
Journal of Medical Microbiology, Society for General Microbiology, 2017, 66 (10), pp.1467-1470. ⟨10.1099/jmm.0.000586⟩
ISSN: 1473-5644
0022-2615
DOI: 10.1099/jmm.0.000586
Popis: International audience; Bird fancier's lung (BFL) is a pulmonary disease caused by inhalation of avian proteins. The involvement of the microorganisms of droppings has been assumed in the past and this idea still persists today. Our study aimed to compare by immunoprecipitation assay the detection of antibodies against both droppings and microorganisms in the sera of patients (n=15) and asymptomatic exposed controls (n=18). We found that 14/15 BFL patients had negative serological results for isolated microorganisms of the droppings, only one positive against Enterobacter sakasakii. Serological arguments were in accordance with diagnosis in 87 % of cases by testing à la carte antigens from each bird dropping versus 20 % using the standard antigenic panel. Otherwise, the microorganisms antigens issued from dropping flora were negative in 93 % of cases. Consequently, it's preferable to use the total extract from the patient's bird droppings to establish the serodiagnosis of the disease.
Databáze: OpenAIRE
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