Microbacterium enclense sp. nov., isolated from sediment sample
| Autor: | Syed G. Dastager, Vidya V. Sonalkar, Rahul Mawlankar, Krishnamurthi Srinivasan, Ashish Verma, Poonam Mual, Meghana N. Thorat |
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| Rok vydání: | 2015 |
| Předmět: |
DNA
Bacterial Geologic Sediments Microbacterium Molecular Sequence Data India Diamino acid Peptidoglycan Biology medicine.disease_cause Microbiology chemistry.chemical_compound Cell Wall RNA Ribosomal 16S Actinomycetales medicine Seawater Ecology Evolution Behavior and Systematics Phospholipids Phylogeny Islands Base Composition Phylogenetic tree Strain (chemistry) Microbacterium enclense Fatty Acids Nucleic Acid Hybridization Vitamin K 2 General Medicine Sequence Analysis DNA biology.organism_classification 16S ribosomal RNA chemistry Bacteria |
| Zdroj: | International journal of systematic and evolutionary microbiology. 65(7) |
| ISSN: | 1466-5034 |
| Popis: | A novel bacterium (strain NIO-1002T) belonging to the genus Microbacterium was isolated from a marine sediment sample in Chorao Island, Goa Province, India. Its morphology, physiology, biochemical features and 16S rRNA gene sequence were characterized. Cells of this strain were Gram-stain-positive, non-motile, non-spore-forming rods that formed yellow-pigmented colonies. It grew in 0–12 % (w/v) NaCl and at 25–37 °C, with optimal growth at 30 °C. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain NIO-1002T is associated with members of the genus Microbacterium, with highest sequence similarity with Microbacterium hominis CIP 105731T (98.1 %) and Microbacterium testaceum KCTC 9103T (98.0 %). Within the phylogenetic tree, this novel strain shared a branching point with M. hominis CIP 105731T. The DNA G+C content was 66.5 mol% and DNA–DNA hybridization relatedness between NIO-1002T, M. hominis CIP 105731T and M. testaceum KCTC 9103T was 39.0 ± 2.0 % and 41.0 ± 2.0 %, respectively. The major fatty acids were ai-C15 : 0, i-C16 : 0 and ai-C17 : 0 and the diamino acid in the cell-wall peptidoglycan of NIO-1002T was lysine. Data obtained from DNA–DNA hybridization and chemotaxonomic phenotypic analysis support the conclusion that strain NIO-1002T represents a novel species within the genus Microbacterium. The name Microbacterium enclense sp. nov. is proposed, with NIO-1002T ( = NCIM 5454T = DSM 25125T = CCTCC AB 2011120T) as the type strain. |
| Databáze: | OpenAIRE |
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