A sugarcane mosaic virus vector for rapid in planta screening of proteins that inhibit the growth of insect herbivores

Autor:	Georg Jander, Barry Chan, Ryan R. Lappe, Mahdiyeh Bigham, Seung Ho Chung, Savithramma P. Dinesh-Kumar, Ugrappa Nagalakshmi, Steven A. Whitham
Rok vydání:	2021
Předmět:	0106 biological sciences 0301 basic medicine Technology Potyvirus Myzus persicae Plant Science Spodoptera maize Medical and Health Sciences Insect Control Zea mays fall armyworm 01 natural sciences Microbiology Hemolysin Proteins 03 medical and health sciences Bacterial Proteins Bacillus thuringiensis Genetics Animals Herbivory Research Articles sugarcane mosaic virus Aphid Genetically modified maize biology venom toxins Rhopalosiphum maidis food and beverages Spodoptera frugiperda Biological Sciences Plants Genetically Modified biology.organism_classification lectins Endotoxins Transformation (genetics) 030104 developmental biology Sugarcane mosaic virus Fall armyworm peptide elicitors Agronomy and Crop Science Research Article 010606 plant biology & botany Biotechnology
Zdroj:	Plant Biotechnology Journal Plant biotechnology journal, vol 19, iss 9
ISSN:	1467-7652 1467-7644
DOI:	10.1111/pbi.13585
Popis:	Spodoptera frugiperda (fall armyworm) is a notorious pest that threatens maize production worldwide. Current control measures involve the use of chemical insecticides and transgenic maize expressing Bacillus thuringiensis (Bt) toxins. Although additional transgenes have confirmed insecticidal activity, limited research has been conducted in maize, at least partially due to the technical difficulty of maize transformation. Here, we describe implementation of a sugarcane mosaic virus (SCMV) vector for rapidly testing the efficacy of both endogenous maize genes and heterologous genes from other organisms for the control of S.frugiperda in maize. Four categories of proteins were tested using the SCMV vector: (i) maize defence signalling proteins: peptide elicitors (Pep1 and Pep3) and jasmonate acid conjugating enzymes (JAR1a and JAR1b); (ii) maize defensive proteins: the previously identified ribosome-inactivating protein (RIP2) and maize proteinase inhibitor (MPI), and two proteins with predicted but unconfirmed anti-insect activities, an antimicrobial peptide (AMP) and a lectin (JAC1); (iii) lectins from other plant species: Allium cepa agglutinin (ACA) and Galanthus nivalis agglutinin (GNA); and (iv) scorpion and spider toxins: peptides from Urodacus yaschenkoi (UyCT3 and UyCT5) and Hadronyche versuta (Hvt). In most cases, S.frugiperda larval growth was reduced by transient SCMV-mediated overexpression of genes encoding these proteins. Additionally, experiments with a subset of the SCMV-expressed genes showed effectiveness against two aphid species, Rhopalosiphum maidis (corn leaf aphid) and Myzus persicae (green peach aphid). Together, these results demonstrate that SCMV vectors are a rapid screening method for testing the efficacy and insecticidal activity of candidate genes in maize.
Databáze:	OpenAIRE
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