Evaluierung des Potenzials von neuralen Stammzellen als tumoradressierende Vehikel für Nanopartikel
Autor: | Baazaoui, Fatima |
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Přispěvatelé: | Mottaghy, Felix M., Clusmann, Hans |
Jazyk: | němčina |
Rok vydání: | 2022 |
Předmět: | |
Zdroj: | Aachen : RWTH Aachen University 1 Online-Ressource : Illustration, Diagramme (2022). doi:10.18154/RWTH-2022-09890 = Dissertation, Rheinisch-Westfälische Technische Hochschule Aachen, 2022 |
DOI: | 10.18154/rwth-2022-09890 |
Popis: | Dissertation, Rheinisch-Westfälische Technische Hochschule Aachen, 2022; Aachen : RWTH Aachen University 1 Online-Ressource : Illustration, Diagramme (2022). = Dissertation, Rheinisch-Westfälische Technische Hochschule Aachen, 2022 The therapy of glioblastoma still poses a major challenge for medicine today. Many innovative approaches are being investigated to enable targeted and effective therapy and thus achieve a better prognosis for affected patients. Neural stem cells and their tumour-migrating properties can be used as a powerful tool in this regard. This work shows that neural stem cells of the ReNcell CX cell line can be effectively loaded with different PEG-based nanogels while maintaining their ability to migrate. The nucleoside analogue EdC was efficiently taken up and incorporated into DNA by glioblastoma cells but poorly by physiological astrocytes and NSC. EdC bound to nanogels via a redox-sensitive linker can be cleaved and incorporated into DNA by glioblastoma cells. The proposed therapeutic approach using NSCs as a transport medium seems very promising. Following further investigations, the use of the radiolabelled nucleoside analogue 125/123ITdU instead of EdC can then be examined in vitro and in vivo. Published by RWTH Aachen University, Aachen |
Databáze: | OpenAIRE |
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