Melatonin Improves Mitochondrial Dynamics and Function in the Kidney of Zücker Diabetic Fatty Rats
| Autor: | Gumersindo Fernández-Vázquez, Russel J. Reiter, Mohamed Tassi, J.M.R. Ferrer, Ahmad Agil, Miguel Navarro-Alarcón, Lucia Visiedo, Meriem Chayah |
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| Přispěvatelé: | [Agil,A, Chayah,M, Visiedo,L] Department of Pharmacology and Neurosciences Institute, School of Medicine, University of Granada, Granada, Spain. [Agil,A, Visiedo,L] Biosanitary Research Institute of Granada (ibs.GRANADA), University Hospital of Granada, Granada, Spain. [Navarro-Alarcon,M] Department of Nutrition and Bromatology, School of Pharmacy, University of Granada, Granada, Spain. [Rodríguez Ferrer,JM] Department of Physiology, School of Medicine, University of Granada, Granada, Spain. [Tassi,M] Service of Microscopy, CIBM, University of Granada, Granada, Spain. [Reiter,RJ] Department of Cellular and Structural Biology, University of Texas Health Science at San Antonio, San Antonio, USA. [Fernández-Vázquez,G] Service of Endocrinology, La Paz Hospital, Madrid, Spain., This work was partially supported by grant SAF2016-79794-R from the Ministerio de Economía y Competitividad (Spain). |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
obesity
MFN2 Obesidad lcsh:Medicine melatonin Mitochondrion Opa1 Melatonina Chemicals and Drugs::Chemical Actions and Uses::Pharmacologic Actions::Molecular Mechanisms of Pharmacological Action::Antioxidants [Medical Subject Headings] 0302 clinical medicine ZDF rats Organisms::Eukaryota::Animals [Medical Subject Headings] Melatonin 0303 health sciences Kidney diabetes Diabetes Diseases::Male Urogenital Diseases::Urologic Diseases::Kidney Diseases [Medical Subject Headings] fission/fusion General Medicine Proteínas quinasas asociadas a muerte celular Phenomena and Processes::Physical Phenomena::Elementary Particles::Electrons [Medical Subject Headings] medicine.anatomical_structure Fission/fusion Proteínas Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Mitochondrial Turnover::Mitochondrial Dynamics [Medical Subject Headings] Chemicals and Drugs::Heterocyclic Compounds::Heterocyclic Compounds 1-Ring::Azoles::Imidazoles::Creatinine [Medical Subject Headings] nephropathy Mitochondrial fission medicine.drug medicine.medical_specialty Renal function Check Tags::Male [Medical Subject Headings] Drp1 Article Nephropathy 03 medical and health sciences Internal medicine Diabetes mellitus medicine Obesity 030304 developmental biology business.industry lcsh:R Chemicals and Drugs::Amino Acids Peptides and Proteins::Proteins::DNA-Binding Proteins::Receptors Cytoplasmic and Nuclear::Receptors Melatonin [Medical Subject Headings] medicine.disease mitochondrial function/dynamic Diseases::Nutritional and Metabolic Diseases::Nutrition Disorders::Overnutrition::Obesity [Medical Subject Headings] Mfn2 Diseases::Pathological Conditions Signs and Symptoms::Signs and Symptoms::Urological Manifestations::Proteinuria [Medical Subject Headings] Endocrinology Nefropatías diabéticas Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Rodentia::Muridae::Murinae::Rats [Medical Subject Headings] Mitochondrial function/dynamic Dinámicas mitocondriales business 030217 neurology & neurosurgery |
| Zdroj: | Digibug. Repositorio Institucional de la Universidad de Granada instname Journal of Clinical Medicine, Vol 9, Iss 2916, p 2916 (2020) Digibug: Repositorio Institucional de la Universidad de Granada Universidad de Granada (UGR) Journal of Clinical Medicine Volume 9 Issue 9 |
| Popis: | Obesity and associated diabetes (diabesity) impair kidney mitochondrial dynamics by augmenting fission and diminishing fusion, which results in mitochondrial and renal dysfunction. Based on available evidence, the antioxidant activities of melatonin may improve impaired renal mitochondrial function in obese diabetic animals by restoring the imbalanced dynamics through inhibiting fission and promoting fusion. Male Zücker diabetic fatty (ZDF) rats and lean littermates (ZL) were orally treated either with melatonin (10 mg/kg BW/day) (M-ZDF and M-ZL) or vehicle (C-ZDF and C-ZL) for 17 weeks. Kidney function was evaluated by measurement of total urine volume, proteinuria, creatinine clearance, and assessment of kidney mitochondrial dynamics and function. C-ZDF exhibited impaired dynamics and function of kidney mitochondria in comparison to C-ZL. Melatonin improved nephropathy of ZDF rats and modulated their mitochondrial dynamics by reducing expression of Drp1 fission marker and increasing that of fusion markers, Mfn2 and Opa1. Furthermore, melatonin ameliorated mitochondrial dysfunction by increasing respiratory control index and electron transfer chain complex IV activity. In addition, it lowered mitochondrial oxidative status. Our findings show that melatonin supplementation improves nephropathy likely via modulation of the mitochondrial fission/fusion balance and function in ZDF rats. Ministerio de Economia y Competitividad (Spain) SAF2016-79794-R |
| Databáze: | OpenAIRE |
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