Improved method for the isolation of RNA from (standing liquid cultures of) Streptomycetes
| Autor: | Krzysztof P. Rembacz, Magdalena Tomczynska, Jeroen Siebring, Geertje van Keulen, Martin Hoogeveen, Lubbert Dijkhuizen |
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| Přispěvatelé: | Faculty of Science and Engineering, Host-Microbe Interactions, Moleculaire Genetica, Groningen Biomolecular Sciences and Biotechnology |
| Rok vydání: | 2004 |
| Předmět: |
Microbiology (medical)
Lysis Efficient lysis Improved method Biology Microbiology Streptomyces Guanidines Isothiocyanates Deoxyribonuclease I GeneralLiterature_REFERENCE(e.g. dictionaries encyclopedias glossaries) Molecular Biology Mycelium Spores Bacterial Phenol fungi RNA Isolation (microbiology) biology.organism_classification Blotting Northern Spore RNA isolation RNA Bacterial Biochemistry ComputingMethodologies_DOCUMENTANDTEXTPROCESSING RNA extraction |
| Zdroj: | Journal of microbiological methods, 58(1), 139-142. ELSEVIER SCIENCE BV |
| ISSN: | 0167-7012 |
| Popis: | Streptomycetes are complex soil bacteria capable of producing aerial reproductive mycelium and secondary metabolites. We observed novel phenomena such as an extended life cycle including flotation and anaerobiosis using standing liquid cultures. This paper describes an improved method for isolating good quality RNA from standing liquid cultures of S. coelicolor via excellent cell lysis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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