Mature ferredoxin-NADP reductase with a glutaminyl residue at N-terminus from spinach chloroplasts
| Autor: | Naoko Sakihama, Masateru Shin, Izumi Nishimura, Shigehiro Obata |
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| Rok vydání: | 1995 |
| Předmět: |
inorganic chemicals
Molecular mass Protein primary structure macromolecular substances Cell Biology Plant Science General Medicine Reductase Biology environment and public health Biochemistry N-terminus enzymes and coenzymes (carbohydrates) Affinity chromatography bacteria Polyacrylamide gel electrophoresis Ferredoxin—NADP(+) reductase Ferredoxin |
| Zdroj: | Photosynthesis Research. 46:323-328 |
| ISSN: | 1573-5079 0166-8595 |
| DOI: | 10.1007/bf00020447 |
| Popis: | When 35%-acetone extract of spinach chloroplasts was separated by SDS-PAGE, ferredoxin-NADP reductase (FNR) appeared as a single band at a molecular mass of 35 kDa. After the polypeptides on the SDS-PAGE plate were electroblotted onto PVDF membrane, the FNR band was cut out and analyzed for N-terminal structure in a gas-phase protein sequencer. Two different FNR peptides were identified: one with glutamine at its N-terminus (Gln-FNR) and the other with γ-pyroglutamic acid ( |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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