Closed vitrification system and egg donation: Predictive factors of oocyte survival and pregnancy

Autor: Margaux Anav, C. Brunet, Vanessa Loup-Cabaniols, Samir Hamamah, Sophie Brouillet, Alice Fournier, Sophie Bringer-Deutsch, Noemie Ranisavljevic, Alice Ferrieres Hoa, Anna Gala
Přispěvatelé: Développement Embryonnaire, Fertilité et Environnement (DEFE), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM), Hôpital Arnaud de Villeneuve [CHRU Montpellier], Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier), Développement embryonnaire précoce humain et pluripotence EmbryoPluripotency (UMR 1203), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)-CHU Montpellier, CCSD, Accord Elsevier
Rok vydání: 2019
Předmět:
Adult
medicine.medical_specialty
Cell Survival
MESH: Tissue Preservation
Reproductive medicine
Context (language use)
[SDV.MHEP.GEO]Life Sciences [q-bio]/Human health and pathology/Gynecology and obstetrics
Oocyte vitrification
MESH: Oocytes
Andrology
03 medical and health sciences
Egg donation
0302 clinical medicine
Human fertilization
MESH: Pregnancy
Pregnancy
medicine
Humans
Vitrification
Survival rate
[SDV.BDLR] Life Sciences [q-bio]/Reproductive Biology
Retrospective Studies
Closed system
030219 obstetrics & reproductive medicine
MESH: Humans
Oocyte Donation
business.industry
Obstetrics and Gynecology
MESH: Adult
MESH: Retrospective Studies
[SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology
medicine.disease
Oocyte
3. Good health
[SDV.MHEP.GEO] Life Sciences [q-bio]/Human health and pathology/Gynecology and obstetrics
medicine.anatomical_structure
Reproductive Medicine
MESH: Cell Survival
030220 oncology & carcinogenesis
MESH: Vitrification
Oocytes
Female
MESH: Oocyte Donation
Tissue Preservation
business
MESH: Female
Zdroj: Journal of Gynecology Obstetrics and Human Reproduction
Journal of Gynecology Obstetrics and Human Reproduction, Elsevier, 2020, 49 (3), pp.101687. ⟨10.1016/j.jogoh.2020.101687⟩
ISSN: 2468-7847
DOI: 10.1016/j.jogoh.2020.101687⟩
Popis: International audience; Although many studies have demonstrated the superiority of ultra-fast freezing compared with slow freezing, the debate is still ongoing concerning the best type of vitrification method: direct exposure to liquid nitrogen (i.e., open systems), or sterile system without contact with liquid nitrogen (i.e., closed systems). The aims of this study were to share our experience on closed vitrification systems in the framework of our egg donation programme with fully asynchronous cycles, and to identify predictive factors of successful outcome in this context. Logistic regression analysis indicated that the number of vitrified oocytes was the only factor predictive of the oocyte survival rate and of clinical pregnancy. The addition of one vitrified oocyte increased by 15 % the odds of oocyte survival. When the oocyte survival rate was considered as a continuous variable, the following results were obtained: 7 % of clinical pregnancy probability for 50 % survival rate, 15 % for 75 % survival rate, and 32 % for 100 % survival rate. The rates of oocyte survival and fertilization, embryo implantation, and clinical pregnancy were in agreement with the recommended values established by ALPHA Scientists in Reproductive Medicine in 2012. On the basis of these results, and according to the European directives on safety, we validate the routine use of closed oocyte vitrification systems for egg donation programmes. These results must be confirmed in larger samples before extrapolation to all patient types.
Databáze: OpenAIRE
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