| Autor: |
Jacob G Hodge, Jennifer L Robinson, Adam J Mellott |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
Regenerative medicine. 18(1) |
| ISSN: |
1746-076X |
| Popis: |
Aim: To compare the physiological behavior of mesenchymal stem/stromal cells (MSCs) within an expandable tissue-mimetic 3D system relative to in vitro expansion in a traditional 2D system. Methods: Adipose-derived MSCs (ASCs) were continuously cultured for 6 weeks on either 2D culture plastic or in a 3D hydrogel system that eliminated subculturing. ASCs were assessed for senescence, ‘stem-like’ MSC markers, and ability for their secretome to augment a secondary cell population. Results: The 3D hydrogel system resulted in an enhanced retention of more regenerative, nonsenescent ASC populations that exhibited increased expression of ‘stem-like’ MSC surface markers. Conclusion: This study introduces a proof-of-concept design for a novel modular 3D system that can improve in vitro expansion of stem-like cell populations for future regenerative therapies. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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