Berberine ameliorates intestinal mucosal barrier dysfunction in nonalcoholic fatty liver disease (NAFLD) rats
| Autor: | Hongtao Hou, Yuzhen Wang, Pingping Li, Yueqin Li, Suxian Cui, Jimin Zheng |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
medicine.medical_specialty
Berberine Blood lipids 02 engineering and technology 010501 environmental sciences 01 natural sciences chemistry.chemical_compound Intestinal mucosa Internal medicine Nonalcoholic fatty liver disease Medicine lcsh:Science (General) 0105 earth and related environmental sciences Multidisciplinary Intestinal permeability Triglyceride business.industry 021001 nanoscience & nanotechnology medicine.disease Intestinal mucosal barrier Endocrinology medicine.anatomical_structure chemistry Hepatocyte Liver function Steatosis 0210 nano-technology business lcsh:Q1-390 |
| Zdroj: | Journal of King Saud University: Science, Vol 32, Iss 5, Pp 2534-2539 (2020) |
| ISSN: | 1018-3647 |
| Popis: | Objective To study the protective role of berberine (BBR) against nonalcoholic fatty liver disease (NAFLD) on intestinal barrier via investigating its effect on intestinal permeability and intestinal innate immune system in a rat model. Method Sprague-Dawley rats were randomly divided into three groups: control rats (group N), high-fat diet (HFD) model rats (group M) and BBR-treated rats (group B). Rats in group M and group B were fed with HFD for 12 weeks to induce NAFLD. Rats in group B were then received 4 weeks of BBR administration with continuous HFD feeding. Samples were collected at 16th week. Results HFD feeding increased the body weight of rats and caused liver steatosis as indicated by hematoxylin & eosin (H&E) staining. Analysis of serum parameters showed that alanine aminotransferase (ALT), aspartate aminotransferase (AST), total cholesterol (TC), triglyceride (TG), endotoxin, interleukin-1β (IL-1β), interleukin-18 (IL-18), and tumor necrosis factor-α (TNF-α) were significantly higher in group M as compared group N. These results confirmed the successful establishment of NAFLD in rats. With 4 weeks of BBR administration, body weight of group B decreased significantly as compared with group M. Serum levels of ALT, AST, TC, TG, endotoxin, IL-1β, IL-18 and TNF-α reduced significantly and hepatocyte steatosis ameliorated. RT-PCR and Western blot analysis showed that BBR reduced the elevated mRNA and protein expressions of innate immune response elements NOD1, NOD2 and NLRP3 that were caused by HFD. BBR also antagonized the effect of NAFLD on Caspase-1 and Claudin-4 protein expressions. Conclusions BBR alleviates endotoxemia, reduces serum lipids, increases liver function, reduces systemic inflammation and diminishes liver inflammation and steatosis in NAFLD rats. The protective effect of BBR against NALFD is achieved through ameliorating intestinal mucosal barrier dysfunction partly by improving permeability of intestinal mucosa and modulating intestinal innate immune components. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|