Characterization of the fine specificity of bovine CD8 T-cell responses to defined antigens from the protozoan parasite Theileria parva
| Autor: | Sarah C. Gilbert, Victor Riitho, Julius Osaso, Fredrick O. Onono, Roger Pelle, Duncan Mwangi, Evans L. N. Taracha, Elias Awino, John K. Nyanjui, W. Ivan Morrison, Jean-Christophe Audonnet, Yoshikazu Honda, Declan J. McKeever, Evelyne Abuya, Mat Yamage, Niall D. MacHugh, Anthony M. Muthiani, Pierre van der Bruggen, Cecelia Muriuki, Ramadhan S. Mwakubambanya, Simon P. Graham, Ferdinand Mbwika, Etienne P. de Villiers, James Gachanja, Shirley A. Ellis, Rosemary Saya |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Male
Cellular immunity Theileria parva Immunology Molecular Sequence Data Epitopes T-Lymphocyte Antigens Protozoan CD8-Positive T-Lymphocytes Major histocompatibility complex Microbiology Epitope Antigen Animals biology Immunodominant Epitopes Histocompatibility Antigens Class I Sequence Analysis DNA MHC restriction biology.organism_classification Virology Epitope mapping Infectious Diseases Microbial Immunity and Vaccines biology.protein Parasitology Cattle Female CD8 Epitope Mapping |
| Zdroj: | Graham, S P, Pelle, R, Yamage, M, Mwangi, D M, Honda, Y, Mwakubambanya, R S, de Villiers, E P, Abuya, E, Awino, E, Gachanja, J, Mbwika, F, Muthiani, A M, Muriuki, C, Nyanjui, J K, Onono, F O, Osaso, J, Riitho, V, Saya, R M, Ellis, S A, McKeever, D J, MacHugh, N D, Gilbert, S C, Audonnet, J C, Morrison, W I, van der Bruggen, P & Taracha, E L 2008, ' Characterization of the fine specificity of bovine CD8 T-cell responses to defined antigens from the protozoan parasite Theileria parva ', Infection and Immunity, vol. 76, no. 2, pp. 685-694 . https://doi.org/10.1128/IAI.01244-07 |
| DOI: | 10.1128/IAI.01244-07 |
| Popis: | Immunity against the bovine intracellular protozoan parasiteTheileria parvahas been shown to be mediated by CD8 T cells. Six antigens targeted by CD8 T cells fromT. parva-immune cattle of different major histocompatibility complex (MHC) genotypes have been identified, raising the prospect of developing a subunit vaccine. To facilitate further dissection of the specificity of protective CD8 T-cell responses and to assist in the assessment of responses to vaccination, we set out to identify the epitopes recognized in theseT. parvaantigens and their MHC restriction elements. Nine epitopes in sixT. parvaantigens, together with their respective MHC restriction elements, were successfully identified. Five of the cytotoxic-T-lymphocyte epitopes were found to be restricted by products of previously described alleles, and four were restricted by four novel restriction elements. Analyses of CD8 T-cell responses to five of the epitopes in groups of cattle carrying the defined restriction elements and immunized with live parasites demonstrated that, with one exception, the epitopes were consistently recognized by animals of the respective genotypes. The analysis of responses was extended to animals immunized with multiple antigens delivered in separate vaccine constructs. Specific CD8 T-cell responses were detected in 19 of 24 immunized cattle. All responder cattle mounted responses specific for antigens for which they carried an identified restriction element. By contrast, only 8 of 19 responder cattle displayed a response to antigens for which they did not carry an identified restriction element. These data demonstrate that the identified antigens are inherently dominant in animals with the corresponding MHC genotypes. |
| Databáze: | OpenAIRE |
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