Upregulation of miRNA-23a-3p rescues high glucose-induced cell apoptosis and proliferation inhibition in cardiomyocytes
| Autor: | Ke Cai, Shuchun Li, Youhua Wang, Yonghao Gui, Yiting Gui, Qian Yang, Yuanzheng Zheng, Jialing Zhang, Qiang Li, Fang Wu, Yawen Zhang, Lian Liu, Feng Wang, Xu Wang |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Cell Proliferation Apoptosis Article Cell Line 03 medical and health sciences 0302 clinical medicine Downregulation and upregulation medicine Animals Myocytes Cardiac H9c2 Cell Proliferation miRNA-23a-3p Chemistry Cell growth Cell Biology General Medicine Transfection Cell cycle Cell biology Rats Up-Regulation MicroRNAs 030104 developmental biology medicine.anatomical_structure Glucose Cell culture 030220 oncology & carcinogenesis Myocardial injury Hyperglycemia Stem cell Developmental Biology |
| Zdroj: | In Vitro Cellular & Developmental Biology. Animal |
| ISSN: | 1543-706X |
| Popis: | Maternal hyperglycemia potentially inhibits the development of the fetal heart by suppressing cardiomyocyte proliferation and promoting apoptosis. Different studies have indicated that miRNAs are key regulators of cardiomyocyte proliferation, differentiation, and apoptosis and play a protective role in a variety of cardiovascular diseases. However, the biological function of miRNA-23a in hyperglycemia-related cardiomyocyte injury is not fully understood. The present study investigated the effect of miRNA-23a-3p on cell proliferation and apoptosis in a myocardial injury model induced by high glucose. H9c2 cardiomyocytes were exposed to high glucose to establish an in vitro myocardial injury model and then transfected with miRNA-23a-3p mimics. After miRNA-23a-3p transfection, lens-free microscopy was used to dynamically monitor cell numbers and confluence and calculate the cell cycle duration. CCK-8 and EdU incorporation assays were performed to detect cell proliferation. Flow cytometry was used to measured cell apoptosis. Upregulation of miRNA-23a-3p significantly alleviated high glucose-induced cell apoptosis and cell proliferation inhibition (p p p Ccna2, Ccne1, and Bcl-2 was downregulated by high glucose and upregulated by miRNA-23a-3p overexpression in high glucose-injured H9c2 cells. miRNA-23a-3p mimics transfection before high glucose treatment had a significantly greater benefit than transfection after high glucose treatment (p |
| Databáze: | OpenAIRE |
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