Residual effect of growth regulators in etiolation and regeneration of in vitro pineapple plants
| Autor: | Maria Angélica Pereira de Carvalho Costa, Antônio da Silva Souza, Ana Maria Mascarenhas Eloy Canto, Fernanda Vidigal Duarte Souza |
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| Rok vydání: | 2010 |
| Předmět: |
chemistry.chemical_classification
micropropagation Regeneration (biology) nodal segments Plant Science germplasm conservation Biology Plant regulators In vitro chemistry.chemical_compound Horticulture plant regulators chemistry Micropropagation Auxin in vitro culture Botany Etiolation Ananas comosus var. comosus Agronomy and Crop Science Gibberellic acid Food Science Hybrid |
| Zdroj: | Revista Brasileira de Fruticultura v.32 n.2 2010 Revista brasileira de fruticultura Sociedade Brasileira de Fruticultura (SBF) instacron:SBFRU |
| ISSN: | 1806-9967 0100-2945 |
| DOI: | 10.1590/s0100-29452010005000075 |
| Popis: | This work aimed to evaluate the influence of naphthaleneacetic acid (NAA) and gibberellic acid (GA3) plant regulators in in vitro etiolation and subsequent regeneration of the PE x SC-60 pineapple hybrid. Nodal segments of in vitro plants with approximately 5-7 cm height were incubated in basic MS culture medium supplemented with 0.0; 0.5 and 1.0 mg L-1 of naphthaleneacetic acid (NAA) in combination with gibberellic acid (GA3) in concentrations of 0.0; 0.5 and 1.0 mg L-1, and maintained at 27 ºC under dark condition. Evaluations were carried out at 90 and 180 days after incubation period. The best results for length of etiolated stems were obtained with 1.0 mg L-1 of NAA. In the experiment followed by the regeneration, stems with 3 cm from the etiolation treatment, were cultivated in proliferation medium and the number of regenerated plants per treatment was evaluated at 60 days of cultivation. The treatment that promoted the best etiolation of plants also promoted the worst regeneration rates, demonstrating the residual effect of the auxin used in the previous step in the regeneration of plants of the pineapple hybrid evaluated. |
| Databáze: | OpenAIRE |
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