Flowing cells through pulsed electric fields efficiently purges stem cell preparations of contaminating myeloma cells while preserving stem cell function
| Autor: | Ana Limón, Neil P. Rodrigues, Abie Craiu, Paul G. Richardson, Joseph A. Mangano, Henry M. Eppich, Rebecca Rich, Eyal C. Attar, Peter S. Choi, Kenneth C. Anderson, David T. Scadden, Paula G. O'Connor, Douglas P. Olson, David Dombkowski, John G. Gribben, Kenneth S. Cohen, Gregor B. Adams, Yoriko Saito, Jonathan Grogins, Jay Freshman, Robert L. Schlossman |
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| Rok vydání: | 2004 |
| Předmět: |
Pathology
medicine.medical_specialty Immunology Tumor Purging Cell Separation Biology Biochemistry Transplantation Autologous Mice Autologous stem-cell transplantation Bone Marrow Mice Inbred NOD medicine Animals Humans Leukapheresis Progenitor cell Cell Size Bone Marrow Purging Hematopoietic Stem Cell Transplantation Cell Biology Hematology Hematopoietic Stem Cells Transplantation Haematopoiesis medicine.anatomical_structure Blood Electroporation Cancer research Bone marrow Stem cell Multiple Myeloma Cytometry |
| Zdroj: | Blood. 105(5) |
| ISSN: | 0006-4971 |
| Popis: | Autologous stem cell transplantation, in the setting of hematologic malignancies such as lymphoma, improves disease-free survival if the graft has undergone tumor purging. Here we show that flowing hematopoietic cells through pulsed electric fields (PEFs) effectively purges myeloma cells without sacrificing functional stem cells. Electric fields can induce irreversible cell membrane pores in direct relation to cell diameter, an effect we exploit in a flowing system appropriate for clinical scale. Multiple myeloma (MM) cell lines admixed with human bone marrow (BM) or peripheral blood (PB) cells were passed through PEFs at 1.35 kV/cm to 1.4 kV/cm, resulting in 3- to 4-log tumor cell depletion by flow cytometry and 4.5- to 6-log depletion by tumor regrowth cultures. Samples from patients with MM gave similar results by cytometry. Stem cell engraftment into nonobese diabetic–severe combined immunodeficient (NOD/SCID)/β2m-/- mice was unperturbed by PEFs. Flowing cells through PEFs is a promising technology for rapid tumor cell purging of clinical progenitor cell preparations. |
| Databáze: | OpenAIRE |
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