Effect of ethanol induced mild stress on post-thawed bull sperm quality
| Autor: | Mahdi Zhandi, Hossein Vaseghi Dodaran, Elaheh Nejati-Amiri, M. A. M. M. Shehab-El-Deen, Mohsen Sharafi, Ardeshir Nejati-Javaremi, Malak Shakeri, Abdollah Mohammadi-Sangcheshmeh |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Male
endocrine system animal structures Motility Apoptosis Semen Biology General Biochemistry Genetics and Molecular Biology law.invention Andrology chemistry.chemical_compound law Mild stress Malondialdehyde Freezing Animals Humans reproductive and urinary physiology Sperm motility Cryopreservation Ethanol urogenital system Cell Membrane Extender General Medicine Spermatozoa Sperm Mitochondria Semen Analysis chemistry Sperm Motility Cattle General Agricultural and Biological Sciences Semen Preservation |
| Zdroj: | Cryobiology. 71:12-17 |
| ISSN: | 0011-2240 |
| DOI: | 10.1016/j.cryobiol.2015.06.008 |
| Popis: | This study was performed to investigate the effect of sub-lethal exposure of bull semen to ethanol on the post-thaw spermatozoa quality. Semen samples (n=24, 6 ejaculates/bull) from 4 Holstein bulls were collected and pooled. Pooled samples were divided into 4 equal parts and each part was frozen after being diluted with Optidyl® extender containing 0 (O-E0), 0.03 (O-E3), 0.09 (O-E9) and 0.15 (O-E15) % (v/v) absolute ethanol. Sperm motility and velocity, plasma membrane integrity and functionality, mitochondrial activity, malondialdehyde concentration, and apoptosis status were evaluated after thawing. A higher percentage of total motility was observed in the O-E9 group as compared to the O-E0, O-E3 and O-E15 groups (p0.05). Also, plasma membrane integrity was higher (p0.05) in the O-E9 group compared to the O-E3, and O-E15 groups. However, the difference between the O-E9 and O-E0 groups was not significant (p0.05). In terms of the proportion of sperm abnormality and plasma membrane functionality no differences (p0.05) were observed between the groups. Our results revealed that malondialdehyde level was lower in ethanol treated (O-E3, O-E9 and O-E15) groups compared to the O-E0 group (p0.05). Furthermore, the percentage of live spermatozoa with active mitochondria was higher in the O-E9 and O-E15 groups compared to the O-E0 and O-E3 groups (p0.05). The O-E3 and O-E9 groups resulted in the highest and lowest percentage of apoptotic spermatozoa, respectively (p0.05). The results of this study demonstrate that supplementation of semen extender with sub-lethal concentration of ethanol influences post-thawed bull sperm quality in a dose dependent manner. |
| Databáze: | OpenAIRE |
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