Endometrial leukocytes are altered numerically and functionally in women with implantation defects
| Autor: | Julie A. DeLoia, Joel S. Krasnow, Jill Brekosky, Ann M. Stewart-Akers |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
medicine.medical_specialty
Transcription Genetic medicine.medical_treatment T-Lymphocytes Immunology Population Cytokine Expression Profile Biology Endometrium Hyperthyroidism Polymerase Chain Reaction Autoimmune Diseases Hypothyroidism Internal medicine medicine Leukocytes Immunology and Allergy Humans Interferon gamma Embryo Implantation education Interleukin 4 education.field_of_study Histocompatibility Antigens Class II Obstetrics and Gynecology Interleukin Immunohistochemistry Thyroid Diseases Interleukin 10 Endocrinology Cytokine medicine.anatomical_structure Reproductive Medicine Cytokines Female Infertility Female medicine.drug |
| Zdroj: | American journal of reproductive immunology (New York, N.Y. : 1989). 39(1) |
| ISSN: | 1046-7408 |
| Popis: | PROBLEM: A significant cohort of women with autoimmune thyroid disease (ATD) also suffer from reduced fertility. The finding that neither exogenous hormones nor donor eggs correct the infertility suggests that the problem involves an inherent endometrial defect. METHOD OF STUDY: Endometrial leukocyte populations in women with ATD were quantitated by immunohistochemistry. A semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR) technique was used to examine the expression of transforming growth factor (TGF)-β1, interleukin (IL)-4, IL-10, and interferon (IFN)-γ in the endometrial samples. RESULTS: A significant increase in the endometrial T-cell population was observed in women with ATD compared to controls. The relative abundance of IL-4 and IL-10 was decreased in women with ATD compared to controls, whereas IFN-γ was increased. No difference was noted in the abundance of TGF-β1. The source of cytokine production for IL-4, IL-10, and IFNγ was the endometrial leukocytes. CONCLUSIONS: Both the leukocyte numbers and cytokine expression profile were altered significantly in a well-defined group of women with implantation defects. |
| Databáze: | OpenAIRE |
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