Identification of the cyclin D1b mRNA variant in mouse
| Autor: | Jack Wu, D. Joshua Liao, Si Hung Wu, Aliccia Bollig, Archana Thakur |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
biology
Reverse Transcriptase Polymerase Chain Reaction Cyclin D Cyclin A Molecular Sequence Data General Medicine Cell cycle Molecular biology Article Cell Line Mice Cyclin D1 Rapid amplification of cDNA ends Gene Expression Regulation Cyclin-dependent kinase Genetics biology.protein Cyclin-dependent kinase complex Animals Protein Isoforms Amino Acid Sequence RNA Messenger Molecular Biology Cyclin A2 |
| Zdroj: | Molecular biology reports. 36(5) |
| ISSN: | 1573-4978 |
| Popis: | Cyclin D1 plays a key regulatory role during the G1 phase of the cell cycle and its gene is amplified and over-expressed in many cancers. The cyclin D1b mRNA variant was established in human cells and recent functional analyses revealed that its protein product harbors unique activities in human cancer cells. By performing reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) experiments, we identified the cyclin D1b mRNA variant in mouse. Similar to its human counterpart, the mouse cyclin D1b transcript consists of exon 1, 2, 3, 4 and part of intron 4, and contains a long open reading frame (ORF). The predicted peptide from this ORF is 34-amino acid longer than the human cyclin D1b. The expression of this mouse mRNA variant was investigated. It appears to be expressed ubiquitously and differentially in various mouse cell lines and tissues and its level might be proportional to that of the canonical endogenous cyclin D1a mRNA. |
| Databáze: | OpenAIRE |
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