Using DNA Metabarcoding to Identify Floral Visitation by Pollinators
| Autor: | Simon Creer, Laura Jones, Abigail Lowe, Natasha De Vere, Lucy Witter |
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| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: | |
| Zdroj: | Lowe, A, Jones, L, Witter, L, Creer, S & de Vere, N 2022, ' Using DNA Metabarcoding to Identify Floral Visitation by Pollinators ', Diversity, vol. 14, no. 4, 236 . https://doi.org/10.3390/d14040236 |
| DOI: | 10.3390/d14040236 |
| Popis: | The identification of floral visitation by pollinators provides an opportunity to improve our understanding of the fine-scale ecological interactions between plants and pollinators, contributing to biodiversity conservation and promoting ecosystem health. In this review we outline the various methods which can be used to identify floral visitation, providing a comparison between molecular and non-molecular methods. We review the literature covering the ways in which DNA metabarcoding has been used to answer ecological questions relating to plant use by pollinators and discuss the findings of this research. We present detailed methodological considerations for each step of the metabarcoding workflow, from sampling through to amplification and finally bioinformatic analysis. Detailed guidance is provided to researchers for utilization of these techniques, emphasizing the importance of standardization of methods and improving the reliability of results. Future opportunities and directions of using molecular methods to analyze plant-pollinator interactions are then discussed. |
| Databáze: | OpenAIRE |
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