Chondroitin sulphate of calf knee-joint cartilage
| Autor: | Ian L. Jones, Rudolf Lemperg |
|---|---|
| Rok vydání: | 1975 |
| Předmět: |
Electrophoresis
musculoskeletal diseases Knee Joint Spectrophotometry Infrared Biophysics Uronic acid Fractionation Cetylpyridinium chloride Biochemistry chemistry.chemical_compound Chondroitin sulphate Isomerism Molecular size Papain medicine Animals Molecular Biology Glycosaminoglycans Chromatography Cartilage Hexosamines Sulfuric Acids Articular surface musculoskeletal system Molecular Weight Uronic Acids medicine.anatomical_structure chemistry Organ Specificity Cattle Chondroitin |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - General Subjects. 392:310-318 |
| ISSN: | 0304-4165 |
| DOI: | 10.1016/0304-4165(75)90012-4 |
| Popis: | Chondroitin sulphate from different layers of calf knee-joint cartilage has been isolated and purified. Analysis for hexosamine, uronic acid, sulphate and relative proportions of the 4- and 6-isomers revealed no differences between the layers. However, an increase in the average molecular weight of the chondroitin sulphate was found to correspond with distance from the articular surface. In particular, the average molecular weight in the epiphyseal cartilage was significantly higher than that in the articular cartilage. The chondroitin sulphate of the 40-μm-thick articular surface layer was found to have a higher molecular weight than the rest of the articular part of the cartilage and also to be more polydisperse. These results, regarding molecular size, are in agreement with the cetylpyridinium chloride cellulose microcolumn fractionation patterns. |
| Databáze: | OpenAIRE |
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