Inhibiting TLR4 signaling by linarin for preventing inflammatory response in osteoarthritis
| Autor: | Zijian Yan, Shuaibo Sun, Haixiao Liu, Xinxian Xu, Weihui Qi, Yanlin Chen, Ping Shang, Xiaoyun Pan, Jingdi Zhan |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Lipopolysaccharides
Aging Necrosis Lipopolysaccharide Lymphocyte Antigen 96 Nitric Oxide Synthase Type II Inflammation Pharmacology Nitric Oxide Menisci Tibial Dinoprostone Nitric oxide Mice chemistry.chemical_compound Chondrocytes In vivo medicine Animals Humans TLR4 Glycosides Prostaglandin E2 Receptor Interleukin-6 Tumor Necrosis Factor-alpha linarin NF-kappa B Cell Biology Extracellular Matrix Toll-Like Receptor 4 osteoarthritis Disease Models Animal MD-2 chemistry Cyclooxygenase 2 chondrocyte medicine.symptom Research Paper medicine.drug |
| Zdroj: | Aging (Albany NY) |
| ISSN: | 1945-4589 |
| DOI: | 10.18632/aging.202469 |
| Popis: | Osteoarthritis (OA) is one of the most common degenerative diseases, ultimately leading to long-term joint pain and severe articular malformation. Controlling local chronic inflammation is a crucial strategy for delaying OA development. Linarin is a natural flavonoid glycoside that is widely available in Compositae, Chrysanthemum indicum and Dendrocalamus and processes protective effects in several animal models. The purpose of our work was to study the protective effect of Linarin for OA. Cellular experiments data showed that Linarin suppressed lipopolysaccharide (LPS)-caused the overproduction of nitric oxide (NO), prostaglandin E2 (PGE2), interleukin-6 (IL-6) and tumour necrosis factor-alpha (TNF-α) in chondrocyte. In addition, LPS-stimulated expression of cyclooxygenase-2 (COX-2) and inducible nitric oxide nitrate (iNOS) was decreased by Linarin pre-treatment. Together, Linarin prevented the catabiosis of extracellular matrix caused by LPS. For mechanism, Linarin inhibited the formation of Toll-like receptor 4 (TLR4) / myeloid differentiation protein-2 (MD-2) dipolymer complex and subsequently intervened NF-κB activation. Our mouse DMM model further clarified the protection of Linarin in vivo. In summary, our results suggested that Linarin may be a potential effective agent for OA. |
| Databáze: | OpenAIRE |
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